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This book intends to report the new progress of pseudotyped viruses, including the construction of pseudotyped viruses with different strategies or vectors for most important viruses. Especially for emerging viruses, optimization of the condition and parameters for assay development based on the pseudotyped viruses and widely application as surrogate of authentic virus to study the biological functions of virus, detection of neutralizing antibody, screening viral entry inhibiters, and others. It includes most pseudotyped viruses that have the protein of the target virus on the surface of the parent virus with incomplete genome. The book is likely to be of interest to all researchers in the field of virology, vaccine, and anti-viral drug development and evaluation.