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This book, now in its fourth edition, aims to promote a deeper understanding of the scientific and clinical basis of nuclear medicine and the new directions in medical imaging. The new edition has been revised and updated significantly to reflect recent changes and to ensure that the contents are in line with likely future directions. In addition to that, chapters have been reorganized in order to simplify the contents and to increase the readability. The book starts by providing essential information on general pathophysiology, cell biology and biologic effects of ionizing radiation followed by the mechanisms of radiopharmaceutical localization in different tissues and cells. This is followed by a series of chapters that covers all relevant organ systems presenting the basic knowledge of anatomy, physiology, and pathology and relating them to the clinical utilization of various scintigraphic modalities. The final chapter is devoted to the basis of therapeutic applications of nuclear medicine. The book will prove invaluable to all with an interest in the pathophysiologic basis of Nuclear Medicine, including nuclear medicine professionals, radiologists, surgeons, pediatricians and internal medicine physicians.

This book covers the discovery of molecular biomarkers, the development of laboratory testing techniques and their clinical applications, focusing on basic research to clinical practice. It introduces new and crucial knowledge and ethics of clinical molecular diagnosis. This book emphasizes the applications of clinical molecular diagnostic test on health management, especially from different diseased organs. It lets readers to understand and realize precision healthcare.

Molecular diagnostics are increasingly used to help guide targeted therapy in solid organ tumors and hematologic malignancies. A large proportion of molecular testing is performed on limited-volume samples obtained via minimally invasive techniques, such as fine needle aspiration. Increasingly, cytopathologists play an essential role in this process, both in the triage of specimens during rapid on-site evaluation and in the evaluation of archival samples to determine suitability for ancillary testing. Therefore, it is imperative that practicing cytopathologists stay abreast of up-to-date diagnostic, prognostic, and predictive ancillary tests that can be used on limited cytologic material. This is a challenge since the landscape of known genomic alterations is constantly evolving and the subsequent set of testing options is ever expanding.The proposed text will provide a user-friendly quick-reference handbook to serve as a useful resource for practicing pathologistsand laboratory personnel dealing with, and interested in, this evolving field of molecular cytopathology. Essential components to be presented include: 1) pre-analytic factors that affect sample selection and evaluation; 2) specimen preparation to maximize confidence in results; 3) interpretation of results; 4) potential limitations; and 5) workflow algorithms. In addition, specific disease specific molecular testing details will be outlined to provide the reader with resources for quick reference. All chapters will be written by experts in their fields and will include the most up-to-date scientific and clinical information.Molecular Diagnostics in Cytopathology will be of value to Cytopathologists, Cytotechnologists, Cytotechnology students, Cytopathology fellows, Surgical pathologists, Pathology residents and fellows, Molecular Pathologists, Molecular pathology fellows, Molecular technologists, as well as Translational researchers with an interest in molecular cytopathology.

This volume provides a concise yet comprehensive overview of minimal residual disease (MRD) testing. The text reviews the history of MRD testing, MRD testing for acute lymphoblastic leukemia/lymphoma, molecular diagnostics for MRD analysis in hematopoietic malignancies, the use of'difference from normal'flow cytometry in monitoring AML response, ML-DS for measurable residual disease detection, and advancements in next generation sequencing for detecting MRD. Written by experts in the field, Minimal Residual Disease Testing: Current Innovations and Future Directions is a valuable resource for hematologists, oncologists, pathologists, and radiologists on the variety of technologies available to detect MRD and how best to integrate these platforms into clinical practice.

The books Molecular Diagnostics Part 1 and 2 provide a comprehensive and practical overview of the state-of-the-art molecular biological diagnostic strategies that are being used in a wide variety of disciplines. The editors and experts in their respective fields have combined their knowledge to write these two books. Many years of experience in the development, application and quality control of molecular diagnostic methods is reflected herewith. Molecular Diagnostics Part 1 is dedicated to the theoretical backgrounds of the technologies often applied in molecular diagnostics, in which nucleic acid amplification methods (such as real-time PCR), sequencing and bioinformatics are the basic tools. The assay design and -development, combined with items of trouble-shooting are described in detail. As a foundation of reliable molecular diagnostic assays, the quality control required for validation, implementation and performance of molecular diagnostic assays is thoroughly discussed. This book also provides extensive information for those working with molecular techniques in a wide variety of research applications using conventional and real-time PCR technology, Sanger and high throughput sequencing techniques, and bioinformatics. Molecular Diagnostics Part 2 highlights the applications of the molecular diagnostic methods in the various diagnostic laboratories, comprising:- Clinical microbiology- Clinical chemistry - Clinical genetics- Clinical pathology - Molecular hematopathology- Veterinary health- Plant health- Food safety Both full-colour and well-illustrated books are particularly valuable for students, clinicians, scientists and other professionals who are interested in (designing) molecular diagnostic methods and for those who wish to broaden their knowledge on the current molecular biological revolution. The information in the books highlights the trend ofthe integration of multiple (clinical) disciplines into one universal molecular laboratory.

This book is intended for practicing pathologists and cytopathologists, as well as for pathology trainees and cytotechnicians. It starts with a detailed description of the extremely important pre-analytical phase for molecular testing followed by a presentation of the key tests and their application in different organs, e.g. the lung or thyroid. Step-by-step instructions for the different assays, reporting and clinical integration of the test results are discussed. The authors help the reader to benefit from their experiences by providing a valuable tool for the implementation of these techniques in daily practice.Though the use of molecular techniques is well established in surgical biopsies, to date they are not widely used in connection with cytological material. However, in some fields like lung cancer or aspirates from the pancreas and biliary tract the only available material for diagnosis is the cytological preparation a fact that has created a need for thestandardization of molecular techniques on cytology.

This two volume book set provides a comprehensive and practical overview of the state-of-the-art molecular biological diagnostic strategies that are currently used in a wide variety of disciplines. The volumes cover: Clinical microbiology and virology Clinical chemistry Pathology Veterinary medicine Plant Pathology Food safety The two volumes are written by experts in their respective fields, who have, together with the editors, combined years of experience in the development, application and quality control of molecular diagnostic methods. The first book is devoted to the theory and backgrounds of molecular techniques, amplification technology, next generation sequencing and bioinformatics for molecular laboratory diagnostics. As a fundament of reliable molecular diagnostic assays, the quality control required for validation, implementation and performance of molecular diagnostic assays is extensively discussed. The second book highlights the applications of these methods in the various diagnostic laboratories. These two full-colour well-illustrated volumes are particularly valuable for students, clinicians, scientists and other professionals who are interested in (designing) molecular diagnostic tests and for those who wish to expand their knowledge on the current molecular biological revolution. The extensive information in both books highlights the current trend of the integration of multiple (clinical) disciplines into one universal molecular laboratory.

This book reviews the current applications of molecular tools in cytopathology and provides a concise handbook for those who provide care in this era of personalized medicine. Specifically, the text provides a comprehensive and concise review of the emerging molecular tests available clinically in different subspecialities of diagnostic pathology. It reviews the current data of molecular testing already applied in cytopathology, discusses some of the biomarkers with potential utility in cytopathology in the near future and reviews the technical challenges in applying and validating molecular tools in liquid-based cytologic materials. Molecular Cytopathology will serve as a valuable resource for cytopathologists, cytotechnologists, pathology trainees, and clinicians with an interest in molecular applications in cytopathology.

This issue of the Surgical Pathology Clinics entitled “Molecular Pathology: Predictive, Prognostic, and Diagnostic Markers in Tumors” is being edited by Dr. Lynette Sholl and will cover molecular pathology in a wide array of anatomic locations including, salivary gland, lung, bladder, glioma, endometrium, colon, pancreaticobiliary tract, sarcoma, myeloid neoplasams, and lymphomas.

Molecular Diagnostics: 12 Tests That Changed Everything focuses on specific laboratory tests and emphasizes how the availability of these tests has altered how clinicians treat their patients. Presented as a standard outline, each chapter focuses on a specific molecular test and provides background on the test and its clinical applications. Continuing with some discussion on how the test is done, interpreted, and used clinically, each chapter then concludes with a discussion of how that test has changed the way medicine is practiced with respect to the disease or condition in question.Authored by renowned experts in the field, Molecular Diagnostics: 12 Tests That Changed Everything is a valuable resource for pathologists, pathology residents, laboratory directors, development personnel, lab medicine fellows and those working in the broad area of oncology, infectious disease and genetics.

Notable practitioners describe how laboratory medicine is practiced today and illuminate how it will function tomorrow as the revolutionary advances afforded by molecular diagnostics become increasingly central to effective analysis. Proceeding from a discussion of elementary nucleic acid technology to a review of the more advanced techniques, the distinguished contributors lay the groundwork for a comprehensive understanding of their applications throughout clinical medicine. The result is a detailed description of those molecular technologies currently used in diagnostic laboratories, as well as those that seem particularly promising. Detailed discussions of specific clinical applications include those for cancer, hematological malignancies, cardiovascular disease, and neuromuscular, endocrine, and infectious diseases.

This entirely case-based book covers a broad cross-section of the practical issues frequently encountered in the day-to-day activities of a molecular genetic pathologist. The book is divided into four sections on the principal areas addressed in molecular genetic pathology (MGP): inherited diseases, hematopathology, solid tumors, and infectious diseases. The topics covered by the cases in each section include test selection, qualitative and quantitative laboratory techniques, test interpretation, prognostic and therapeutic considerations, ethical considerations, technical troubleshooting, and result reporting. This book will be ideal for trainees in MGP and clinical molecular genetics who require a practice-based preparation for board examinations. It will also be very useful for residents and fellows in medical specialties to which MGP is pertinent, and for practicing pathologists who want to learn more about the current practice of molecular diagnostics.

This unique book provides a thorough overview of developing molecular cancer diagnostic assays, which are the prerequisites for optimal solutions within personalized cancer medicine. The book takes the reader through definitions of the pharmacodiagnostic concept, historical perspectives of the early steps into molecular cancer diagnostics linked to therapy, the basis of different diagnostic molecular techniques, ongoing research, drug-diagnostic co-development, assay validation, clinical trial methodology, regulatory issues around pharmacodiagnostics and future aspects within personalized cancer medicine.

Taking advantage of the many major advances that have occurred since their groundbreaking first edition was published, William B. Coleman and Gregory J. Tsongalis have updated and expanded their highly praised tutorial guide to molecular diagnostic techniques to include not only improved traditional methods, but also totally new molecular technologies, some not yet in routine use. The authors offer cutting-edge molecular diagnostics for genetic disease, human cancers, infectious diseases, and identity testing, as well as new insights into the question of quality assurance in the molecular diagnostics laboratory. Additional chapters address other technologies found in the clinical laboratory that are complementary to molecular diagnostic methodologies, and also discuss genetic counseling and the ethical and social issues involved with nucleic acid testing. Authoritative and state-of-the-art, Molecular Diagnostics: For the Clinical Laboratorian, 2nd Edition is the essential textbook of choice for anyone working in molecular diagnostics and who wants to remain current with this rapidly changing field.

Radioisotope-based molecular imaging probes provide unprecedented insight into biochemistry and function involved in both normal and disease states of living systems, with unbiased in vivo measurement of regional radiotracer activities offering very high specificity and sensitivity. No other molecular imaging technology including functional magnetic resonance imaging (fMRI) can provide such high sensitivity and specificity at a tracer level. The applications of this technology can be very broad ranging from drug development, pharmacokinetics, clinical investigations, and finally to routine diagnostics in radiology. The design and the development of radiopharmaceuticals for molecular imaging studies using PET/MicroPET or SPECT/MicroSPECT are a unique challenge. This book is intended for a broad audience and written with the main purpose of educating the reader on various aspects including potential clinical utility, limitations of drug development, and regulatory compliance and approvals.

This authoritative textbook offers in-depth coverage of all aspects of molecular pathology practice and embodies the current standard in molecular testing. Since the successful first edition, new sections have been added on pharmacogenetics and genomics, while other sections have been revised and updated to reflect the rapid advances in the field. The result is a superb reference that encompasses molecular biology basics, genetics, inherited cancers, solid tumors, neoplastic hematopathology, infectious diseases, identity testing, HLA typing, laboratory management, genomics and proteomics. Throughout the text, emphasis is placed on the molecular variations being detected, the clinical usefulness of the tests and important clinical and laboratory issues.The second edition of Molecular Pathology in Clinical Practice will be an invaluable source of information for all practicing molecular pathologists and will also be of utility for other pathologists, clinical colleaguesand trainees.

This volume in the Foundations in Diagnostic Pathology Series packs today's most essential cell and tissue base molecular pathology into a compact, high-yield format! It focuses on the state of the art in practical validated molecular diagnostics as applied across the fields of surgical pathology and cytology. With an emphasis on current, clinically valid, and diagnostically important applications today and in the near future, you can be assured you're getting the most up-to-date, authoritative coverage available. Its pragmatic, well-organized approach, nearly 250 full-color illustrations, and at-a-glance boxes and tables make the information you need easy to access. Practical and affordable, this resource is ideal for study and review as well as everyday clinical practice!Offers detailed discussions on today's technologies to help you select the best test for case evaluation. Presents recognized molecular pathologists who convey the most current information, keeping you on the cusp of your field. Features nearly 250 full-color illustrations that present important pathologic features, enabling you to form a differential diagnosis and compare your findings with actual cases.Uses a consistent, user-friendly format, including at-a-glance boxes and tables for easy reference.

Airway inflammation is now recognized as central to the pathophysiology of several major respiratory conditions-the two most common being asthma and chronic obstructive pulmonary disease (COPD). In Human Airway Inflammation: Sampling Techniques and Analytical Protocols, experienced basic and clinical investigators describe a wide range of readily reproducible methods for sampling, isolating, and culturing all of the major inflammatory cells involved in the pathophysiology of asthma and COPD. The collection techniques detailed here involve biopsy and sampling of airway liquids such as sputum and exhaled gases such as nitric oxide. Also provided is a full range of methods for the isolation and characterization of cells and for the measurement of many of the major inflammatory markers, along with protocols for measuring the inflammatory mediators and enzymes released during lung inflammation. Each user-friendly method contains all the essential step-by-step elements the researcher must navigate from sample collection to analysis, and includes notes on how to avoid problems. Among the more advanced techniques are the measurement of exhaled hydrocarbons and F2 isoprostanes, granulocyte pharmacodynamics in whole blood measured by flow cytometry, and tracing mediator trafficking in eosinophils using confocal microscopy. Comprehensive and highly practical, the methods presented in Human Airway Inflammation: Sampling Techniques and Analytical Protocols provide today's basic and clinical researchers with all the major techniques for investigating airway inflammation, and powerfully illuminate many novel targets for emerging drugs.