1. A strategy to identify mixed polysaccharides through analyzing the monosaccharide composition of disaccharides released by graded acid hydrolysis.
- Author
-
Cao, Chunyang, Wang, Lilong, Wang, Linlin, Gong, Yue, Ai, Chunqing, Wen, Chengrong, and Song, Shuang
- Subjects
- *
DISACCHARIDES , *POLYSACCHARIDES , *MONOSACCHARIDES , *MOLECULAR weights , *HYDROLYSIS , *TISSUES - Abstract
• This study has provided disaccharide data of Cyclina sinensis for the first time. • There are mainly 6 disaccharides in Cyclina sinensis. • A potential strategy to identify mixed polysaccharides without purification. It is still a challenge to identify mixed polysaccharides isolated from biological tissues. The present study aimed to develop a protocol that allowed for quick characterization of mixed polysaccharides by selecting Cyclina sinensis polysaccharides as test beds. Disaccharides were gradually released by graded acid hydrolysis, separated with 3 kDa molecular weight cut-off membrane, and analyzed by HPLC-ESI-MSn. Furthermore, the monosaccharide composition of the filtrate during the gradient acid hydrolysis process and the final retentate were also determined to provide composition information of these disaccharides. Thus, 6 disaccharides were detected and identified, indicating the existence of polysaccharides constructed of corresponding repeated disaccharide fragments in C. sinensis. The present study demonstrated a potential strategy to characterize mixed polysaccharides without separation and purification. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF