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A water-soluble heteropolysaccharide (PUP60W-1) was purified from the hot water extract of sclerotia of P. umbellatus by chromatography with DEAE Sepharose Fast Flow and Sephacryl S200 High-Resolution. The primary structure of PUP60W-1 was elucidated by GC, GC–MS and NMR. PUP60W-1 was identified as a highly branched polysaccharide composed of fucose, glucose and galactose in a ratio of 1.0:0.9:13.3. The main repeating unit was identified as α-(1 → 6)- d -galactopyranan backbone with substitution of terminal α-galactopyranosyl residues at O -2 for two out of every three main chain galactose residues. Its chain conformation was studied by atom force microscopy and size exclusion chromatography coupled with multiple detectors. The results revealed that PUP60W-1 had a molecular weight of 2.47 × 10 4 Da with a polydispersity index of 1.04, and existed in water as compact sphere structures which could be disrupted into smaller spherical chain blocks after dispersion with sodium dodecyl sulfate. [ABSTRACT FROM AUTHOR]

Abstract: A novel water-soluble heteropolysaccharide (PEPS1), with a molecular weight of 1.88×104 Da as determined by high performance liquid chromatography (HPLC), specifically size exclusion chromatography, was isolated from the fruiting bodies of Pleurotus eryngii by hot water extraction and further purification by DEAE Sepharose Fast Flow and Sephacryl S-300 and S-100 High-Resolution chromatography. By use of compositional analysis, methylation analysis, together with 1H, 13C NMR and 2D NMR spectroscopy including COSY, TOCSY, HMQC, HMBC and NOESY experiments, the PEPS1 was identified as a heteropolysaccharide that consists of a α-d-(1→6)-Galactopyranan backbone with a β-d-Mannosyl unit on O-2 of the 2,6-di-O-substituted-d-Galactosyl units, α-(1→6)-3-O-Me-d-Galactopyranan backbone with a terminal α-3-O-Me-d-Galactosyl unit and it also contained a minor 2,3,6-α-d-Galatopyranan units and 4-β-d-Mannopyranan residues. [Copyright &y& Elsevier]

Abstract: A novel water-soluble polysaccharide named PGPB-1, with a molecular weight of 1.3×104 Da as determined by high-performance liquid chromatography (HPLC), was isolated from the fruiting bodies of Pleurotus geesteranus by boiling water as well as DEAE-Sepharose Fast Flow and High-Resolution Sephacryl S-300. On the bases of compositional analysis, methylation analysis and NMR spectroscopy (1H NMR, 13C NMR, 2D-COSY, TOCSY, HMQC, HMBC and NOESY spectra) analysis, the structural elucidation of PGPB-1 consisted of a α-d-(1→6)-galactopyranan and a α-d-3-O-Me-d-galactosyl unit backbone with a α-d-Mannosyl unit on O-2 of the 2,6-di-O-substituted-d-galactosyl units. The repeating unit of the polysaccharide was established as: Display Omitted PGPB-1 also contains a minor proportion of 6-linked-d-Glcp. [Copyright &y& Elsevier]

Abstract: A new heteropolysaccharide (HEPF4), with a molecular weight of 2.03×104 Da as determined by high-performance liquid chromatography (HPLC), was obtained from the fruiting bodies of Hericium erinaceus. It is composed of 3-O-methylrhamnose, l-fucose, d-galactose and d-glucose in the ratio of 0.12:1.00:3.27:0.28. Its chemical structure was characterized by sugar and methylation analysis, along with 1H and 13C NMR spectroscopy, including NOESY and HMBC experiments for linkage and sequence analysis. The polysaccharide is composed of a tetrasaccharide repeating unit (described in the results section). HEPF4 also contains a minor proportion of glucose and 3-O-methylrhamnose which is believed to terminate the polymer main chain. [Copyright &y& Elsevier]

Abstract: A novel water-soluble heteropolysaccharide FVP60-B was extracted from the fruiting bodies of Flammulina velutipes with boiling water and purified by Sephacryl S-300 and S-400, which molecular weight was estimated to be 1.3×104 Da by HPLC. It is composed of l-fucose, d-mannose, d-glucose and d-galactose in a ratio of 1.16:0.82:1.00:3.08. Sugar analysis, methylation analysis together with 1H, 13C and 2D NMR spectroscopy disclosed that FVP60-B is consisted of a α-(1→6)-d-galactopyranan backbone with a terminal fucosyl, terminal glucosyl and α-(1→6)-d-mannopyranan units on O-2 of 2,6-O-substituted-d-galactosyl units. [Copyright &y& Elsevier]