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In this work, Cu 2 O/CuO hollow microspheres (Cu x OHM) are scattered on the reduced graphene oxide (RGO) nanosheet to form Cu x OHM/RGO hybrid by a simple one-step hydrothermal method. Characterizations of the prepared Cu x OHM/RGO hybrid were performed by X-ray diffraction, X-ray photoelectron spectroscopy and scanning electron microscopy. Cyclic voltammetry and chronoamperometry were employed to investigate the electrocatalytic properties of the Cu x OHM/RGO hybrid. We found that the constructed glucose sensor has a broad linear range of 0.01−14.0 mM (R2 = 0.997), a low detection limit of 1.15 μM and a high sensitivity of 635.315 μA/mM cm2. In comparison with pristine Cu x OHM and RGO, the prepared hybrid glucose sensor showed significantly enhanced electrocatalytic activity. Additionally, the sensor also shows fabulous anti-interference ability, good stability. The excellent electrocatalytic performance together with the simple and low-cost preparation method make Cu x OHM/RGO hybrid be an appealing electrocatalyst in glucose sensor application. [ABSTRACT FROM AUTHOR]