1. Revealing the Genetic Variation and Allele Heterozygote Javanese and Arab Families in Malang East Java Indonesia.
- Author
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Sari, Nila Kartika, Prawestiningtyas, Eriko, and Fatchiyah, Fatchiyah
- Subjects
- *
HUMAN genetic variation , *ALLELES , *HUMAN genome , *TANDEM repeats , *INFORMED consent (Medical law) - Abstract
Purpose: The purpose of this study is to identify genetic variability the family of Javanese married with Arab and their allele patterns for paternity testing. Material and Methods: We used human white blood cell from three generations of three families, consists of:1 grandmothermother, father-daughter2, grandfather-mother, father-daughter3, grandfather, grandmother - mother, father-son. DNA blood samples were isolated by salting out standard procedure, and amplified by PCR with applying 13 CODIS which consists of TPOX, D3S1358, FGA, D5S818, CSF1PO, D7S820, D8S1179, TH01, VWA, D13S317, D16S539, D18S51, D21S11 and amelogenin primer. The Fingerprint profile was visualized by 8% polyacrylamide gel and took the picture by ChemiDoc gel Imaging and measure the intensity band pattern by Quantity One software Results: Our result showed that the genetic variability and heterozygote allele increasing by using the 13 CODIS markers from the first generation to the next generation with paternity testing from each family were matched. Conclusion: We can conclude that in a Javanese-Arab family ethnic seems stimulate the increasing genetic variation and allele heterozygote. [ABSTRACT FROM AUTHOR]
- Published
- 2014