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A water-soluble fingerprint probe, GK-Ac, was designed and synthesized by using an unsymmetric structure of fluorescent groups. In the present contribution, incorporating a "rotor" structure to impede the rotation of rotational site groups, which can effectively enhance aggregation-induced emission and provide additional sites for modification. The results indicate that the fingerprint probe GK-Ac had faint fluorescence characteristics in aqueous solution, and the level 2 structure of latent fingerprints can be clearly observed at 450 nm fluorescence when soaked in lipid or sprayed with the GK-Ac aqueous solution. Moreover, the probe was also successfully applied to detect the level 3 structure of latent fingerprints with flat surfaces. The results can provide reliable theoretical guidance for the development of fingerprint fluorescence developer. [Display omitted] • An AIE-based water-soluble probe was designed and synthesized by using an unsymmetric structure. • The level 3 features of LFPs can be revealed by soaking or spraying with the GK-Ac. • GK-Ac only binds to lipids on LFPs and does not bind to other substances. [ABSTRACT FROM AUTHOR]

Biothiols play an important role in cell protein metabolism and signal transduction, especially Hcy was considered as a new risk factor closely related to many diseases. However, there remain some limitations in selectively detecting Hcy due to its structural similarity to Cys. It is urgent for us to develop a novel indicator to study the pathological process of Hcy. Herein, we constructed a new fluorescent probe TCF-SBD composed of tricyanofuran fluorophore and SBD reaction site for detecting Hcy. The probe TCF-SBD exhibited a strong yellow emission response to Hcy in an "off-on" manner, and showed good selectivity for Hcy over Cys and GSH. The sensing mechanism was confirmed by HPLC, NMR titration and DFT studies. The probe was sensitive to the detection of Hcy with LOD low to 0.23 μM. The powerful probe TCF-SBD succeeded in real-time and selectively visualizing Hcy in living cells. [Display omitted] • Fluorescence probe based on substitution-rearrangement process was synthesized for selective detection of Hcy. • The probe displayed high selective and sensitive for Hcy (detection limit is 0.23 μM). • The probe was applied for fluorescence imaging of exogenous Hcy in living cells. [ABSTRACT FROM AUTHOR]

A novel spiropyran-based colorimetric probe SP-DNP bearing a 2,4-dinitrophenyl group as a PhSH-responsive site was synthesized for the detection of thiophenol based on the ring open/closed isomerization process. Upon the addition of PhSH, the probe SP-DNP displayed a remarkable change in UV–vis spectra with a good linear relationship to concentration of PhSH, and result in apparent color change from purple to colorless that could be easily observed by the naked eye even in the presence of other interfering anions. Probe SP-DNP exhibited specific colorimetric response toward PhSH with very fast response (within 30 s) and high sensitivity (detection limit is 0.55 μM). In addition, the probe showed good selectivity and sensitivity toward PhSH through fluorescence quenching process. The sensing mechanism of the nucleophilic substitution-cyclization process was confirmed by 1H NMR and mass spectroscopic analysis. Moreover, probe SP-DNP could detect PhSH in real water samples. Image 1 • Probe SP-DNP was constructed on basis of thiophenol-induced transform merocyanine to spiropyran isomerization process. • The probe could colorimetric and fluorescent respond toward thiophenol with fast response and high sensitivity. • The probe has been successfully demonstrated in monitoring thiophenol in real water samples. [ABSTRACT FROM AUTHOR]