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Abstract: A new heteropolysaccharide with a molecular weight of 1.94×104 Da, HEPF1, was isolated from the fruiting bodies of Hericium erinaceus. It is composed of fucose, galactose and glucose in the ratio of 1:4:1, as well as a minor proportion of 3-O-methyl rhamnose. Sugar analyses, methylation analysis, together with 1H and 13C NMR spectroscopy established that HEPF1 has a (1→6)-linked α-d-galactopyranosyl backbone with branches that are composed of fucose attached to O-2; it also contains 6-O-substituted-β-d-oligoglucosyl units and a minor terminal 3-O-methyl rhamnose residue. [Copyright &y& Elsevier]

Abstract: A novel water-soluble heteropolysaccharide termed PBF2 was isolated from the fruiting bodies of Phellinus baumii Pilát using hot water extraction and purified on Sephacryl S-1000. PBF2 was shown to be composed of l-fucose, d-mannose and d-glucose. Its structural characteristics were further investigated by FT-IR spectroscopy, sugar analysis, methylation analysis and NMR spectroscopy. Based on the data obtained, PBF2 was found to be a heteropolysaccharide containing a β-(1→6)-d-glucopyranose backbone with a fucosyl unit on O-3 of the 3,6-di-O-substituted-d-glucosyl units and it also contained a minor (1→3, 6)-β-d-mannose residue and terminal glucose residues. [Copyright &y& Elsevier]

Highlights: [•] It was isolated and purified by columns of DEAE-Sepharose F. F. and Sephacryl S H.R. [•] It was a homogeneous polysaccharide and the molecular weight was estimated to be 1.08×104 Da by HPLC. [•] The composition of it was determined as l-fucose, d-mannose, d-glucose and d-galactose by GC analysis. [•] Its structure was determined by MS, along with 1H, 13C and 2D NMR spectroscopy. [ABSTRACT FROM AUTHOR]