1. Genome-wide identification of the TIFY gene family in tobacco and expression analysis in response to Ralstonia solanacearum infection.
- Author
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Zhang, Huifen, Liu, Zhengwen, Geng, Ruimei, Ren, Min, Cheng, Lirui, Liu, Dan, Jiang, Caihong, Wen, Liuying, Xiao, Zhiliang, and Yang, Aiguo
- Subjects
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BACTERIAL wilt diseases , *GENE families , *RALSTONIA solanacearum , *TOBACCO analysis , *DRUG resistance in bacteria , *PROMOTERS (Genetics) , *TOBACCO - Abstract
The TIFY gene family plays an essential role in plant development and abiotic and biotic stress responses. In this study, genome-wide identification of TIFY members in tobacco and their expression pattern analysis in response to Ralstonia solanacearum infection were performed. A total of 33 TIFY genes were identified, including the TIFY, PPD, ZIM&ZML and JAZ subfamilies. Promoter analysis results indicated that a quantity of light-response, drought-response, SA-response and JA-response cis -elements exist in promoter regions. The TIFY gene family exhibited expansion and possessed gene redundancy resulting from tobacco ploidy change. In addition, most NtTIFY s equivalently expressed in roots, stems and leaves, while NtTIFY1 , NtTIFY4 , NtTIFY18 and NtTIFY30 preferentially expressed in roots. The JAZ III clade showed significant expression changes after inoculation with R. solanacearum , and the expression of NtTIFY7 in resistant varieties, compared with susceptible varieties, was more stably induced. Furthermore, NtTIFY7 -silenced plants, compared with the control plants, were more susceptible to bacterial wilt. These results lay a foundation for exploring the evolutionary history of TIFY gene family and revealing gene function of NtTIFY s in tobacco bacterial wilt resistance. • Thirty-three NtTIFY genes were identified in tobacco and grouped into four subfamilies. • The JAZ III clade was highly induced under the infection of R. solanacearum and treatment with Me-JA. • NtTIFY7 contributes to resistance against bacterial wilt in tobacco. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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