Search

Highlights • The study designed and developed a heteromorphic sample pool. • Modeling method based on 2D light intensity distribution patterns can effectively enhance components detection precision and speed. • The study can provide meaningful and insight guidance for analytical chemistry and high-throughput test. Abstract To enhance the detection precision of samples with scattering characteristics by near infrared spectroscopy (NIRS), this study developed a heteromorphic sample pool and established the related 2D light intensity acquisition system, which can simultaneously acquire multi-path exit light adsorption and scattering information of the samples under test. The Intralipid-20% phantom solutions in 34 samples with different concentrations were detected, while one-dimensional (1D) exit light intensity distributions and two-dimensional (2D) exit light intensity distributions on the surface of the samples were analyzed and modeled using partial least squares. In contrast with the prediction results based on the modeling method of 1D exit light intensity distribution, the modeling method of 2D exit light intensity distribution exhibits more favorable results; specifically, correlation coefficient enhanced by 2.48%, while root mean square error reduced by 6.89%. The experimental results demonstrate that using heteromorphic sample pool can effectively achieve NIRS-based detection precision and speed of chemical components in the solutions with scattering characteristics, which can provide important references for high-throughput and high-precision detection of turbid media in analytical chemistry. [ABSTRACT FROM AUTHOR]