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Homocysteine (Hcy) is detrimental to bone health in a mouse model of diet‐induced hyperhomocysteinemia (HHcy). However, little is known about Hcy‐mediated osteoblast dysfunction via mitochondrial oxidative damage. Hydrogen sulfide (H2S) has potent antioxidant, anti‐inflammatory, and antiapoptotic effects. In this study, we hypothesized that the H2S mediated recovery of osteoblast dysfunction by maintaining mitochondrial biogenesis in Hcy‐treated osteoblast cultures in vitro. MC3T3‐E1 osteoblastic cells were exposed to Hcy treatment in the presence or absence of an H2S donor (NaHS). Cell viability, osteogenic differentiation, reactive oxygen species (ROS) production were determined. Mitochondrial DNA copy number, adenosine triphosphate (ATP) production, and oxygen consumption were also measured. Our results demonstrated that administration of Hcy increases the intracellular Hcy level and decreases intracellular H2S level and expression of the cystathionine β‐synthase/Cystathionine γ‐lyase system, thereby inhibiting osteogenic differentiation. Pretreatment with NaHS attenuated Hcy‐induced mitochondrial toxicity (production of total ROS and mito‐ROS, ratio of mitochondrial fission (DRP‐1)/fusion (Mfn‐2)) and restored ATP production and mitochondrial DNA copy numbers as well as oxygen consumption in the osteoblast as compared with the control, indicating its protective effects against Hcy‐induced mitochondrial toxicity. In addition, NaHS also decreased the release of cytochrome c from the mitochondria to the cytosol, which induces cell apoptosis. Finally, flow cytometry confirmed that NaHS can rescue cells from apoptosis induced by Hcy. Our studies strongly suggest that NaHS has beneficial effects on mitochondrial toxicity, and could be developed as a potential therapeutic agent against HHcy‐induced mitochondrial dysfunction in cultured osteoblasts in vitro. [ABSTRACT FROM AUTHOR]