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Your search keyword '"Jin, Ying"' showing total 2 results
Start Over Author "Jin, Ying" Topic quantitative analysis Journal journal of chromatography b: analytical technologies in the biomedical & life sciences Database Academic Search Index
2 results on '"Jin, Ying"'

1. Profiling post-translational modifications of histones in neural differentiation of embryonic stem cells using liquid chromatography–mass spectrometry.

Author

Zheng, Shuzhen, Sun, Ming, Zhang, Kai, Gu, Junjie, Guo, Zhenchang, Tian, Shanshan, Zhai, Guijin, He, Xiwen, Jin, Ying, and Zhang, Yukui

Abstract

The neural differentiation of embryonic stem cells (ESCs) is of great significance for understanding of the mechanism of diseases. Histone post-translational modifications (HPTMs) play a key role in the regulation of ESCs differentiation. Here, we combined the stable isotope chemical derivatization with nano-HPLC-mass spectrometry (MS) for comprehensive analysis and quantification of histone post-translational modifications (HPTMs) in mouse embryonic stem cells (mESCs) and neural progenitor cells (mNPCs) that was derived from ESCs. We identified 85 core HPTM sites in ESCs and 78HPTM sites in NPCs including some novel lysine modifications. Our quantitative analysis results further revealed the changes of HPTMs from ESCs to NPCs and suggested effect of combinational HPTMs in the differentiation. This study demonstrates that HPLC–MS-based quantitative proteomics has a considerable advantage on quantification of combinational PTMs and expands our understanding of HPTMs in the differentiation. [ABSTRACT FROM AUTHOR]

Published
2016
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2. Profiling post-translational modifications of histones in neural differentiation of embryonic stem cells using liquid chromatography–mass spectrometry.

Author

Zheng, Shuzhen, Sun, Ming, Zhang, Kai, Gu, Junjie, Guo, Zhenchang, Tian, Shanshan, Zhai, Guijin, He, Xiwen, Jin, Ying, and Zhang, Yukui

Subjects
*EMBRYONIC stem cells, *HISTONE genetics, *POST-translational modification, *NEURONS, *CELL differentiation, *LIQUID chromatography-mass spectrometry, *QUANTITATIVE research
Abstract

The neural differentiation of embryonic stem cells (ESCs) is of great significance for understanding of the mechanism of diseases. Histone post-translational modifications (HPTMs) play a key role in the regulation of ESCs differentiation. Here, we combined the stable isotope chemical derivatization with nano-HPLC-mass spectrometry (MS) for comprehensive analysis and quantification of histone post-translational modifications (HPTMs) in mouse embryonic stem cells (mESCs) and neural progenitor cells (mNPCs) that was derived from ESCs. We identified 85 core HPTM sites in ESCs and 78HPTM sites in NPCs including some novel lysine modifications. Our quantitative analysis results further revealed the changes of HPTMs from ESCs to NPCs and suggested effect of combinational HPTMs in the differentiation. This study demonstrates that HPLC–MS-based quantitative proteomics has a considerable advantage on quantification of combinational PTMs and expands our understanding of HPTMs in the differentiation. [ABSTRACT FROM AUTHOR]

Published
2016
Full Text
View/download PDF

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