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The research aim was to determine the effectiveness of disinfection methods: thyme essential oil microatmosphere (TEO), silver nanoparticles misting (AgNPs), and low temperature plasma (LTP) by culture-dependent method and RNA analysis. In addition, we examined the influence of disinfection on mechanical and optical properties of paper from historical books with different levels of microbial contamination. All disinfection methods were generally bacteriostatic and fungistatic. The AgNPs misting method was more effective for bacterial inhibition ( R = 60–100%), than LTP ( R = 25–100%) and TEO ( R = 12–100%); all tested methods were less effective for fungi ( R = 0–99.8%). TEO exhibits a broader spectrum of fungicidal activity compared to AgNPs and LTP. The antimicrobial effectiveness depended on the area from where the microorganisms were isolated and their sensitivity to the active agent. A higher disinfection effectiveness was observed for books with higher levels of microbial contamination. RNA concentration was a good marker of antimicrobial activity of disinfection. RNA amount decreased by 95–100% after disinfection with LTP and TEO, and by 29–89% after AgNPs disinfection. Disinfection of paper from historical books with LTP, AgNPs and TEO did not significantly influence or have a positive effect on the mechanical and optical properties of paper from tested historical books. We show that LTP, TEO, and AgNPs can be used as microbiostatics, alternatively to the currently available methods. [ABSTRACT FROM AUTHOR]

Abstract: The goal of preventive conservation is to provide the correct maintenance of our cultural heritage in order to enrich our future. The Canadian Museum of Nature developed a risk model for preventive conservation that has proven useful in application to a Portuguese archive. This work applies this model to compare the magnitudes of specific risks estimated for this same archive collection when located in either of two pairs of storage rooms. These rooms are in two different parts of this building: two are in an older part and two are in a recent addition. It was, therefore, necessary to assess the building itself, both structurally and environmentally, as well as analyse its common human practices. In terms of the overall risk magnitude, the best room was found to be in the recent building and the worst in the older building. However, risks related to water problems were found to be higher in the new building. In this work cost-free measures and easy to implement recommendations are given in order to improve the quality of the storage rooms. A final comment on the method itself is also given. [Copyright &y& Elsevier]

A new portable nuclear magnetic resonance (NMR) device allows in situ non-invasive investigation of paper in order to examine some aspects of the microscopic healthy state of documents of historical and artistic interest. The apparatus has a NMR surface probe, which permits to perform most of the NMR relaxometric measurements on objects of almost every size and shape. It uses non-ionizing radio frequency electromagnetic waves and is easily transportable without hazard for people or environment. Some results obtained with this device on the pages of the Codex Major, a manuscript musical anthology that belongs to the Collectio Altaempsiana (1600–1610) of Palazzo Altaemps in Rome, are here presented. The NMR results provide indications about the spread of the deterioration process of the paper and of the corrosion effect caused by the iron-gall ink. [Copyright &y& Elsevier]