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Applying lime to ameliorate soil acidity has been observed to induce manganese (Mn) deficiency in canola (Brassica napusL.) crops grown on acid sandy soils near Albany and gravelly acid sands of the Great Southern Districts of southwestern Australia. These soils were often Mn-deficient in patches for wheat (Triticum aestivumL.) production when they were newly cleared for agriculture requiring application of Mn fertilizer to ensure grain yields were not reduced by the deficiency. Since then, these soils have acidified and in the 1990s, canola started to be grown on these soils in rotation with wheat and lupins (Lupinus angustifoliusL.). These limed soils may now have become marginal to deficient in Mn for canola production. The effect of liming may change the effectiveness of fertilizer Mn. In addition, the effect of liming on the residual value of Mn fertilizer applied to these soils for canola production is unknown. Therefore, a glasshouse experiment was conducted using Mn deficient sand. Three levels of finely-powdered calcium carbonate were added and incubated in moist soil for 42 days at 22±2°C to produce 3 soils with different pH values [1:5 soil:0.01 M calcium chloride (CaCl2)]: 4.9 (original soil), 6.3, and 7.5. Five Mn levels, as solutions of Mn sulfate, were then added and incubated in moist soil for 0, 50, and 100 days before sowing canola. To estimate the residual value (RV) of incubated Mn for canola production, the effectiveness of the incubated Mn was calculated relative to the effectiveness of Mn applied just before sowing canola (freshly-applied Mn). The RV of the incubated Mn was determined using yield of dried canola shoots, the Mn application level required to produce 90% of the maximum shoot yield, and Mn content in dried shoots (Mn concentration in shoots multiplied by yield of dried shoots). As measured using both yield of dried shoots and Mn content of dried shoots, the residual value of Mn decreased with increasing soil pH and with increasing period of incubation of Mn with moist soil. The critical Mn concentration, for 90% of the total yield of dried canola shoots, was (mg Mn kg−1) ∼17 in youngest mature growth (apex and youngest emerged leaf, YMG), and ∼22 for the rest of dried shoots. These values were similar to current critical values for un-limed soils suggesting critical Mn concentrations remain the same for limed soils. Plant testing of canola is recommended if soils are to be limed to ameliorate soil acidity. When plant tests indicate a high likelihood of Mn deficiency, foliar Mn sprays need to be applied to that crop to ensure Mn deficiency does not reduce grain production that year, and fertilizer Mn needs to be re-applied to the soil when sowing the next crop to reduce the likelihood of Mn deficiency for subsequent crops. [ABSTRACT FROM AUTHOR]