1. Detection and Characterization of Swine Origin Influenza A(H1N1) Pandemic 2009 Viruses in Humans following Zoonotic Transmission.
- Author
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Cook, Peter W., Stark, Thomas, Jones, Joyce, Kondor, Rebecca, Zanders, Natosha, Benfer, Jeffrey, Scott, Samantha, Yunho Jang, Janas-Martindale, Alicia, Lindstrom, Stephen, Blanton, Lenee, Schiltz, John, Tell, Rachel, Griesser, Richard, Shult, Peter, Reisdorf, Erik, Danz, Tonya, Fry, Alicia, Barnes, John, and Vincent, Amy
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H1N1 influenza , *SWINE influenza , *PANDEMICS , *RANDOM forest algorithms , *INFLUENZA viruses , *SEASONAL influenza - Abstract
Human-to-swine transmission of seasonal influenza viruses has led to sustained human-like influenza viruses circulating in the U.S. swine population. While some reverse zoonotic-origin viruses adapt and become enzootic in swine, nascent reverse zoonoses may result in virus detections that are difficult to classify as "swine-origin" or "human-origin" due to the genetic similarity of circulating viruses. This is the case for human-origin influenza A(H1N1) pandemic 2009 (pdm09) viruses detected in pigs following numerous reverse zoonosis events since the 2009 pandemic. We report the identification of two human infections with A(H1N1)pdm09 viruses originating from swine hosts and classify them as "swine-origin" variant influenza viruses based on phylogenetic analysis and sequence comparison methods. Phylogenetic analyses of viral genomes from two cases revealed these viruses were reassortants containing A(H1N1)pdm09 hemagglutinin (HA) and neuraminidase (NA) genes with genetic combinations derived from the triple reassortant internal gene cassette. Follow-up investigations determined that one individual had direct exposure to swine in the week preceding illness onset, while another did not report swine exposure. The swine-origin A(H1N1) variant cases were resolved by full genome sequence comparison of the variant viruses to swine influenza genomes. However, if reassortment does not result in the acquisition of swine-associated genes and swine virus genomic sequences are not available from the exposure source, future cases may not be discernible. We have developed a pipeline that performs maximum likelihood analyses, a k-mer-based set difference algorithm, and random forest algorithms to identify swine-associated sequences in the hemagglutinin gene to differentiate between human-origin and swine-origin A(H1N1)pdm09 viruses. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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