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Objective: In order to further study the effect of biology behavior of the gene 14-3-3θ to breast cancer, to build a recombinant vector and breast cancer cell line which can over express gene 14-3-3θ. Methods: The gene 14-3-3θ in the pENTR 3C DUAL is synthesized by a company, we put the gene 14-3-3θ into the vector pcDNA4.0 which can product 14-3-3θ in eukaryotic cells by recombinant DNA technology and then transfect the 293T, we identify the overexpression of the recombinant vector by the western blot and PCR. At last, we transfect the breast cancer cell by the recombinant vector and screen the breast cancer cell with Zeocin. Results: Successfully construct the recombinant vector and breast cancer cell line which can over express 14-3-3θ. Which shows that gene 14-3-3θ may play an important role in the early diagnosis and prognosis of breast cancer prediction. [ABSTRACT FROM AUTHOR]