1. Molecular structure and characterization of the cytokine TWEAK and its receptor Fn14 in bovine
- Author
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Zhang, Jia-Xin, Sang, Ming, Li, Jian-Feng, Zhao, Wei, Ma, Hong-Wei, Min, Cui, Hu, Yun-Long, Du, Ming-Xiang, and Zhang, Shuang-Quan
- Subjects
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MOLECULAR structure , *CYTOKINES , *CIRCULAR DNA , *GENETIC code , *BIOINFORMATICS , *PROTEIN structure , *REVERSE transcriptase polymerase chain reaction , *ANIMAL models in research - Abstract
Abstract: Full-length cDNA encoding TWEAK and Fn14 from bovine was isolated. We used bioinformatics to analyze the gene structure, function, evolutionary relationships, and predicted three-dimensional structure of proteins and binding sites. Real-time quantitative PCR (Q-PCR) analysis revealed that both TWEAK and Fn14 are constitutively expressed in various tissues in bovine. Bovine TWEAK and Fn14 interaction analysis by yeast two-hybrid. Our results suggest that the TWEAK–Fn14 pathway is evolutionarily highly conserved. It will be helpful for investigation on the biological role of the TWEAK/Fn14 system in this important animal model. Furthermore, it provides insight into the molecular evolution of the emerging TWEAK and Fn14 families. [Copyright &y& Elsevier]
- Published
- 2011
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