1. A IFI27 gene contributes to ER-stress mediated apoptosis and benefits for white spot syndrome virus infection in Litopenaeus vannamei.
- Author
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Fan, Jin-Quan, Miao, Yu-Tao, Lu, Ke-Cheng, Chen, Guo-liang, Li, Bin-Bin, Hong, Qian-Ming, Yang, Xin-Jun, Yan, Ze-Yu, and Chen, Yi-Hong
- Subjects
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WHITE spot syndrome virus , *WHITELEG shrimp , *VIRUS diseases , *UNFOLDED protein response , *APOPTOSIS - Abstract
The interplay between virus and host has been one of the hot spot in virology, and it is also the important aspect of revealing the mechanism of virus infection. Increasing studies revealed that several key molecules took part in the process of virus-host interaction. White spot syndrome virus (WSSV) has been proved to affect several physiological processes of the host cells, especially apoptosis. While the relationship between them still remains unclear. In this study, a IFI27 gene (LvIFI27) of Litopenaeus vannamei was cloned. It is indicated that LvIFI27 was induced upon endoplasmic reticulum (ER)-stress and unfolded protein response activator Thapsigargin. Unlike human IFI27 locating to mitochondria, LvIFI27 lied to ER, and was involved in cell apoptosis process. Moreover, results of cumulative mortality analysis showed that LvIFI27 might contributed to WSSV proliferation by promoting apoptosis during the process of viral infection. Findings in this study enriched our understanding of the relationship between WSSV infection and ER-stress mediated apoptosis. • Over-expressed LvIFI27 in S2 cells resulted in apoptosis. • Expression of LvIFI27 in L. vannamei hemocytes was induced upon unfolded protein response. • Knockdown expression of LvIFI27 decreased the cumulative mortality of WSSV infection shrimp. • Knockdown expression of LvIFI27 reduced the hemocytes apoptosis rate under white spot syndrome virus infection. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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