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Abstract: A set of twelve simple sequence repeat markers was used to evaluate the genetic background of 738 pomegranate (Punica granatum L.) accessions, which belonged to the Iranian National Pomegranate Collection, from 23 provinces of Iran. Genetic diversity among the accessions was analysed using several variability parameters. Forty-three alleles were detected with a mean of 3.59 alleles per locus. The highest levels of polymorphism were found for the locus ABRII-MP26. The mean values of expected heterozygosity and polymorphic information content were 0.521 and 0.458, respectively. Neighbour-Net analysis based on uncorrected P distances and a model-based method separated the accessions into 8 main groups and several subgroups, with a tendency to cluster by geographical origin. From the simple sequence repeat analysis, it can be concluded that the genetic base of the Iranian National Pomegranate Collection is broad enough to ensure future progress in breeding programmes and these markers are effective tools for conducting genetic diversity studies in pomegranate and developing strategies for germplasm management. [Copyright &y& Elsevier]

Abstract: The full length hemagglutinin (HA) genes of 287 H9N2 AI strains isolated from chickens in Asia during the period 1994–2009 were genetically analyzed. Phylogenetic analysis showed that G1-like viruses circulated in the Middle East and Indian sub-continent countries, whereas other sublineages existed in Far East countries. It also revealed G1-like viruses with an average 96.7% identity clustered into two subgroups largely based on their time of isolation. The Ka/Ks ratio was calculated 0.34 for subgroup 1 and 0.57 for subgroup 2 indicates purifying/stabilizing selection, but despite this there is evidence of localized positive selection when comparing the subgroups 1 and 2 protein sequences. Five sites in HA H9N2 viruses had a posterior probability >0.5 using the Bayesian method, indicating these sites were under positive selection. These sites were found to be associated with the globular head region of HA. To identify sites under positive selection; amino acid substitution classified depends on their radicalism and neutrality. The results indicate that, although most positions in HAs were under purifying selection and can be eliminated, a few positions located in the antigenic regions and receptor binding sites were subject to positive selection. [Copyright &y& Elsevier]

Abstract: A recombinant antigen-based single serum dilution ELISA was developed for simultaneous detection and subtyping of influenza viruses. Recombinant baculovirus encoding the hemagglutinin (HA1 subunit) of H9N2 virus was generated. To evaluate the rHA1-ELISA, microplates were coated with purified HA1 protein and tested with reference control sera. Subsequently, 92 field sera collected from chickens suspected to be infected with H9N2 AIV were employed to test the efficacy of the rHA1-ELISA. The sera were tested simultaneously by HI and a commercial AIV ELISA kit. The rHA1-ELISA appeared to be highly specific and sensitive for direct detection of H9N2 antibodies in serum samples. [ABSTRACT FROM AUTHOR]

In this study, carbon nanofibers (CNFs) assisted chitosan (Cs) was utilized to surface modification of poly(ether sulphone) (PES) nanofiltration membranes. The effect of CNFs-co-Cs nanocomposite layer on physical/chemical properties and separation performance of prepared membranes was investigated. The ability of membranes in heavy metal ions removal from water was studied. Scanning electron microscopy (SEM), Scanning optical microscopy (SOM), Fourier transform infrared spectroscopy (FTIR) and 3D surface images were employed for the membranes characterization. Moreover membrane porosity, mean pore size, water content, water contact angle, flux, salt rejection and tensile strength of fabricated membranes measured. The FTIR analysis verified formation of CNFs-co-Cs layer on the membrane surface. SOM images revealed relatively uniform surface for the prepared membrane. Introducing of CNFs-co-Cs on the PES substrate led to decline of water contact angle from 62.66° to 43.86° sharply. Modified membranes showed higher porosity and water content than virgin PES membrane. The Na 2 SO 4 rejection was measured >66% for PES membrane while it was >82% for PES/CNFs-co-Cs membrane containing of 0.5 wt% CNFs. Pure water flux also enhanced from 4.25 (L/m2.h) for virgin PES membrane up to 13.58 (L/m2.h) for the surface modified membranes. Mechanical tensile strength was also increased considerably for the PES/CNFs-co-Cs membrane containing of 0.5 wt% CNFs that were 3.5 times more than the tensile strength for PES membrane. The Cu, Cr and Pb rejections measured 97.86%, 98.40% and 96.54% for the PES/CNFs-co-Cs membrane with 0.5 wt % CNFs while they were 45.3%, 48.46% and 38.02 for PES substrate respectively. [Display omitted] • PES/CNFs-co-Cs nanofiltration membrane fabricated through intra-networking. • PWF measured 4.25 and 13.58 (L/m 2 h) for PES and [PES/CNFs-co-Cs] membranes. • Na 2 SO4 rejection was >66% and >88% for virgin and surface modified membranes. • [PES/CNFs-co-Cs] membrane showed high ability in Cu, Cr, Pb removal from water. • Outstanding tensile strength obtained for CNFs-co-Cs coated PES based NF membrane. [ABSTRACT FROM AUTHOR]

Summary: In spite of reports on cytokines induction by the Brucella DNA in murine model, there is no comparison between pathogenic and appropriate vaccine strains in human. We investigated the cytokines profile of human peripheral blood mononuclear cells (PBMCs) induced by DNA extracted from pathogenic isolates of Brucella melitensis and B. abortus as well as Rev1 and S19; the appropriate vaccine strains. It was observed that despite differential induction of Interleukin(IL)-12 and IL-10 production, identical IL-12/IL-10 concentration ratio was obtained by all Brucella strains DNAs that was 2 after 24h and 4 after 5 days of incubation. In addition, IL-2 and Interferon(IFN)-γ production were profoundly increased compared to the medium at day 3 and 5 respectively but IFN-α was not induced. Therefore, Brucella strains DNAs are Th1 inducing component with similar pattern in human PBMCs. [Copyright &y& Elsevier]

Chemoresistance is often referred to as a major leading reason for cancer therapy failure, causing cancer relapse and further metastasis. As a result, an urgent need has been raised to reach a full comprehension of chemoresistance-associated molecular pathways, thereby designing new therapy methods. Many of metastatic tumor masses are found to be related with a viral cause. Although combined therapy is perceived as the model role therapy in such cases, chemoresistant features, which is more common in viral carcinogenesis, often get into way of this kind of therapy, minimizing the chance of survival. Some investigations indicate that the infecting virus dominates other leading factors, i.e., genetic alternations and tumor microenvironment, in development of cancer cell chemoresistance. Herein, we have gathered the available evidence on the mechanisms under which oncogenic viruses cause drug-resistance in chemotherapy. [Display omitted] [ABSTRACT FROM AUTHOR]

Therapeutic vaccines are an effective approach in cancer therapy for treating the disease at later stages. The Food and Drug Administration (FDA) recently approved the first therapeutic cancer vaccine, and further studies are ongoing in clinical trials. These are expected to result in the future development of vaccines with relatively improved efficacy. Several vaccination approaches are being studied in pre-clinical and clinical trials, including the generation of anti-cancer vaccines by plant expression systems.This approach has advantages, such as high safety and low costs, especially for the synthesis of recombinant proteins. Nevertheless, the development of anti-cancer vaccines in plants is faced with some technical obstacles.Herein, we summarize some vaccines that have been used in cancer therapy, with an emphasis on plant-based vaccines. [Display omitted] [ABSTRACT FROM AUTHOR]

• The most IL-6 and TNF-α gene expression was observed in full flagellin adjuvanted group. • The recombinant full flagellin adjuvanted group showed the highest cellular activity. • The highest HI titer was observed in commercial vaccine and recombinant flagellin adjuvanted groups. The present study was designed to investigate the potential application of native (N) and recombinant (truncated modified [tmFliC] and full-length [flFliC]) flagellin proteins along with inactivated Newcastle disease virus (NDV). Fifty six SPF chickens were immunized twice with PBS (control), inactivated NDV (Ag), inactivated NDV/flFliC (AgF), inactivated NDV/tmFliC (AgT), inactivated NDV/N (AgN), commercial vaccine containing Montanide (Vac) and Vac/N (VacN), with a two-week interval. Blood was collected weekly and spleens were harvested after chickens were sacrificed. Interleukin-6 (IL-6) and tumor necrotic factor-α (TNF-α) gene expression in peripheral blood mononuclear cells were analyzed by Real-Time PCR. Antibody response was assessed by haemagglutination inhibition (HI). Cellular activity was quantified by MTT assay. Results showed that the most IL-6 and TNF-α gene expression was observed in AgF group (P < 0.01). The lowest gene expression among vaccinated groups was observed in Ag group for IL-6 and Ag and Vac group for TNF-α. The highest HI titer was observed in Vac, VacN, AgF and AgT groups. The AgF group showed the highest cellular activity (P < 0.01). In conclusion, flagellin-adjuvanted groups showed a pro-inflammatory effect and acted similarly to or better than the Vac group. Hence, flagellin can be proposed as a potential adjuvant for ND vaccine. [ABSTRACT FROM AUTHOR]

• Bacterial infections in serious burns have been a constant threat to human health throughout history. • Chitosan has significant antibacterial activity against a broad spectrum of bacteria. • Chitosan nanoparticles could be used as drug carriers and gene carrier to enhance their transfer efficiency in the cells. • Chitosan-based nanomaterials could be used against bacterial infections. Chitosan nanomaterials have become a hot topic in biomedicine due to exerting antimicrobial effects with interestingly high levels of biodegradability and biocompatibility without causing toxicity. Regarded as a potential means of wound dressing with antimicrobial activity, chitosan exhibits higher efficiency when it is functionally modified with other natural compounds, metallic antimicrobial particles and antibiotics. Mechanistically, the antibacterial effect of chitosan is mostly, associated with the death-proceeding leakage of intracellular content, induced by malfunction and altered permeability of the negatively charged cell membrane, on which chitosan is adsorbed. Moreover, chitosan nanoparticles (NPs) are endowed with favorable features of NPs (i.e., large surface-to-volume ratio, high functionalization possibilities and a greater capacity for drug loading), as well as that of their chitosan base, thereby possessing strengthened antibacterial potential. In addition, polycations target negatively charged bacterial membranes, so bacteria cells are more strongly affected by polycationic chitosan NPs than pure chitosan. [ABSTRACT FROM AUTHOR]

• Sleep deprivation impaired Learning and memory in Barnes and Novel location tasks. • Sleep deprivation disrupted Synaptic plasticity. • Prior voluntary exercise modulated impairments resulting from subsequent SD. Previous studies have indicated that forced exercise plays a preventive role in synaptic plasticity deficits in the hippocampus and behavioral impairments in sleep-deprived male and female rats. The objective of the present study was to evaluate the effects of voluntary exercise on early long-term potentiation (E-LTP) at the Cornu Ammonis (CA1) area of the hippocampus and behavioral functions by barnes maze and novel location tests in sleep-deprived female rats. Intact female Wistar rats were used in the present study. The exercise protocol was four weeks wheel running and the multiple platform method was applied to induce 72 h Sleep deprivation (SD). We examine the effect of exercise and/or SD on synaptic plasticity using in vivo extracellular recording in the CA1 area of the hippocampus. Spatial learning and memory examined by Barnes maze and recognition memory assessed by novel location test. Field potential recording indicated that the induction and maintenance phase of E-LTP impaired in the sleep deprived animals compared to the other groups. After 72 h SD, LTP impairments were reduced by 4 weeks of voluntary exercise but do not go back to control values. SD impairs learning and memory and exercise could improve these deficits. In conclusion, the synaptic plasticity deficit in sleep-deprived female rats was improved by voluntary exercise. Further studies are suggested to evaluate the possible underlying mechanisms. [ABSTRACT FROM AUTHOR]

Background: Metabolic syndrome involves a set of metabolic risk factors that directly increases the risk of atherosclerotic cardiovascular disease. Physical inactivity due to driving can increase the risk of metabolic syndrome. It is also known that sleep disorders (sleep apnea) can result in MetS. Driving in Iran is considered a very popular but risky occupation, so paying attention to this profession is of special importance. Therefore, the researchers aimed to investigate the association between sleep disorders and metabolic syndrome in drivers in Shahroud city in 2018.Methods: This cross-sectional study was carried out on 948 drivers from Shahroud city in 2018. After obtaining consent from participants, 3 questionnaires including demographic, Epworth Sleepiness Scale and STOP-BANG were completed. Clinical and anthropometric measurements were assessed, including blood pressure, waist circumference, hip circumference, weight, height, and body mass index. In addition, blood was drawn to measure High Density Lipoprotein, Low Density Lipoprotein, Triglyceride, cholesterol, and Fasting Blood Sugar levels. The relationship between metabolic syndrome and sleep disorders was then studied. In this study, statistical analyses were performed using SPSS software version 23 at a significance level of 0.05.Results: Mean age of drivers was 44.15 ± 11.66 (years). The mean waist circumference and mean hip circumference in subjects with a Class 1 Driver's License (a certificate for trucks and buses) were higher than those with a Class 2 Driver's License (a certificate for motorcars, minibuses, vans, etc. (seating< 20)) (P = 0.01 and P = 0.003, respectively). Moreover, the BMI in subjects with a Class 1 Driver's License was higher compared to subjects with a Class 2 Driver's License. The correlation between metabolic syndrome with sleep apnea based on STOP-BANG questionnaire was significant (p < 0.001) irrespective of definition (ATP and IDF).Conclusions: According to the results of this study, there was a bi-directional association between sleep disorders and Mets, so this group should pass periodic medical examinations and training courses. Moreover, their families should be informed of prevention and treatment of this syndrome. [ABSTRACT FROM AUTHOR]

• Chronic pain is highly comorbid with PTSD symptoms in war veterans. • Attentional bias in pain is suggested to be relevant to anxiety in chronic pain. • Veterans showed a greater attentional bias away from pain-related cues. • Avoidance from pain may indicate suppression of emotion in stressful situations. • Veterans showed significantly elevated attentional bias variability for both happy and pain faces compared to controls. Cognitive models propose that attentional dysregulation, including an attentional bias towards threat, is one of the factors through which chronic pain and post-traumatic stress symptoms (PTSS) maintain and exacerbate one another. The current investigation assessed the attentional bias for painful facial expressions and its relationship with PTSS, using both traditional and variability-based attentional bias measures, among veterans with chronic pain and PTSS and controls. Fifty-four veterans with chronic pain and 30 age/education-matched controls participated in this investigation. Participants completed a self-report measure of PTSS and a modified version of the dot-probe task with painful, happy, and neutral facial expressions. Attention was assessed using both traditional and variability-based reaction time measures of attentional bias. Veterans directed attention away from painful facial expressions (i.e., avoidance) relative to both the control group (between-subject effect) and relative to neutral faces (within-subject effect). Veterans also showed significantly elevated attentional bias variability for both happy and painful facial expressions compared to controls. Attentional bias variability for happy and painful facial expressions was correlated with PTSS among all participants. Veterans with chronic pain and PTSS avoided pain-related stimuli and displayed an overall attentional dysregulation for emotional facial expressions. Avoidance of pain cues may be a coping strategy that these individuals develop under stressful conditions. Implications, limitations, and directions for future research are discussed. [ABSTRACT FROM AUTHOR]

Background: One of the most important causative agents of visceral leishmaniasis (VL) is Leishmania infantum, which is mainly spread by Phlebotomus and Lutzomyia sandflies in the Old and New World, respectively. Novel and effective drugs to manage this neglected vector-borne disease are urgently required. In this study, we evaluated the toxicity of carvacrol, thymol and linalool, three common essential oil constituents, on amastigotes and promastigotes of L. infantum. Methods: in vitro experiments were performed by 24 h MTT assay. Carvacrol, thymol and linalool at concentrations ranging from 1.3 to 10 μg/mL were tested on promastigotes of L. infantum. For in vivo test, two groups of hamsters (Mesocricetus auratus) received 100 mg/kg of body weight/day of carvacrol and thymol as intraperitoneal injection on day 7 post-infection, followed by a 48 h later injection. The third group was treated with the glucantime as standard drug (500 mg/kg) and the last group (control) just received normal saline. On the 16th day, the number of parasites and histopathological changes in liver and spleen were investigated. Results: 24 h MTT assay showed promising antileishmanial activity of thymol and carvacrol, with IC50 values of 7.2 (48 μM) and 9.8 μg/mL (65 μM), respectively. Linalool at all concentrations did not affect L. infantum promastigote viability. In vivo toxicity data of carvacrol and thymol showed that the former at 100 mg/kg was the safest and most effective treatment with little side effects on the liver. Conclusions: Overall, thymol and carvacrol are highly promising candidates for the development of effective and safe drugs in the fight against VL. [ABSTRACT FROM AUTHOR]