1. Deciphering the human cellular interactors of alphavirus unique domain of chikungunya virus.
- Author
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Ghildiyal, Ritu and Gabrani, Reema
- Subjects
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CHIKUNGUNYA virus , *PROTEIN-protein interactions , *COMPLEMENTARY DNA , *VIRAL proteins , *HYBRID systems , *ALPHAVIRUSES , *ALPHAVIRUS diseases - Abstract
• AUD is a multifunctional domain of nsP3 essential for Chikungunya virus life cycle. • The human interactors of CHIKV AUD were identified utilizing yeast two hybrid system. • SNAPIN and N4BP2L2 interaction with CHIKV AUD was validated by pull down assay. • Key residues involved in SNAPIN and N4BP2L2 association with AUD were predicted. The life-threatening re-emerged chikungunya virus (CHIKV) can cause an epidemic outbreak and still has no vaccine available so far. Alphavirus unique domain (AUD) of CHIKV nsP3 is a multifunctional domain that remains conserved among alphaviruses and is critical for CHIKV replication. The understanding of AUD-host protein-protein interactions and their association with the cellular processes concerning CHIKV infection are not well studied. In the current study, the protein-protein interactions of AUD and its human host were elucidated by screening of universal human cDNA library using yeast two-hybrid system. The chosen interactions were further validated by GST pull-down assay, and their network mapping was analyzed. The study revealed that the identified interactors are linked with the vesicle trafficking and transcription corepressor activities. Further, the interfacial residues of interactions between viral and host proteins were predicted, which will further provide the new platform to develop novel antivirals. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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