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High-speed counter-current chromatography (HSCCC) was successfully used for the separation of abietane-type diterpenoids from the medicinal plantC. kaichianum, which were not separated in our previous study using preparative HPLC. The HSCCC separation employed the lower phases ofn-hexane–ethyl acetate–methanol–water (HEMW) 4:5:4:5 and HEMW 4:5:5:4 as the mobile phase for stepwise elution while the upper phase of HEMW 4:5:4:5 was used as the stationary phase. HSCCC separation yielded 90.5 mg of compound1(kaichianone A), 137.7 mg of compound2(kaichianone B), 125.0 mg of compound3(teuvincenone E), and 227.6 mg of compound4(taxusabietane A) with purities of 95.3%, 97.2%, 97.8%, and 98.6%, respectively, as determined by HPLC. Compounds1–2are two new abietane-type diterpenoids while Compounds3–4are known abietane-type diterpenoids, analyzed by ESIMS and NMR data. The results demonstrated that HSCCC can be an excellent alternative for other separation methods. The two new compounds showed significant cytotoxicity against ileocecal carcinoma HCT-8 and breast adenocarcinoma MCF-7 cells. High-speed counter-current chromatography (HSCCC) was successfully used for the separation of abietane-type diterpenoids from the medicinal plantC. kaichianum, which were not separated in our previous study using preparative HPLC. [ABSTRACT FROM PUBLISHER]