1. Dietary extra virgin olive oil attenuates kidney injury in pristane-induced SLE model via activation of HO-1/Nrf-2 antioxidant pathway and suppression of JAK/STAT, NF-κB and MAPK activation.
- Author
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Aparicio-Soto, Marina, Sánchez-Hidalgo, Marina, Cárdeno, Ana, Rosillo, María Ángeles, Sánchez-Fidalgo, Susana, Utrilla, Jose, Martín-Lacave, Inés, and Alarcón-de-la-Lastra, Catalina
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SYSTEMIC lupus erythematosus , *AUTOIMMUNE diseases , *OLIVE oil , *MITOGEN-activated protein kinases , *NF-kappa B , *PROTEIN metabolism , *ENZYME metabolism , *ANIMAL experimentation , *ANIMALS , *ANTIOXIDANTS , *BIOCHEMISTRY , *BIOLOGICAL models , *CELLULAR signal transduction , *CYTOKINES , *KIDNEY diseases , *PHENOMENOLOGY , *MICE , *OXIDOREDUCTASES , *DNA-binding proteins , *DISEASE complications - Abstract
Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by a widespread organ involvement. Recent studies have suggested that extra virgin olive oil (EVOO) might possess preventive effects on this immunoinflammation-related disease. However, its role in SLE remained unknown. In this work, we evaluated the effects of EVOO diet in a pristane-induced SLE model in mice. Three-month-old mice received an injection of pristane or saline solution and were fed with different experimental diets: sunflower oil diet or EVOO diet. After 24weeks, mice were sacrificed, spleens were collected and kidneys were removed for immunoinflammatory detections. The kidney expression of microsomal prostaglandin E synthase 1, heme oxygenase 1 (HO-1), nuclear factor E2-related factor 2 (Nrf-2), mitogen-activated protein kinases (MAPKs), Janus kinase/signal transducer and activator of transcription (JAK/STAT) and nuclear transcription factor-kappa B (NF-κB) pathways were studied by western blotting. In addition to macroscopic and histological analyses, serum matrix metalloproteinase 3 (MMP-3) levels and proinflammatory cytokines production in splenocytes were evaluated by enzyme-linked immunoassay. We have demonstrated that EVOO diet significantly reduced renal damage and decreased MMP-3 serum and PGE2 kidney levels as well as the proinflammatory cytokines production in splenocytes. Our data indicate that Nrf-2 and HO-1 protein expressions were up-regulated in those mice fed with EVOO and the activation of JAK/STAT, MAPK and NF-κB pathways were drastically ameliorated. These results support the interest of EVOO as a beneficial functional food exerting a preventive/palliative role in the management of SLE. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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