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Current evidence supports the idea that animal domestication occurred in multiple loci and at different time periods throughout the Near East rather than according to a single origin. In this scenario, the southern Levant is being increasingly considered as another presumed primary centre of goat domestication, alongside eastern Anatolia and the Zagros. Assessing the status (i.e. wild or domestic) of this animal in this region, however, is hampered by the difficulty of morphologically distinguishing domestic goats from their wild relatives. Diet reconstruction is a powerful approach to address this issue since the feeding of managed animals is partially controlled by their owners, unlike free-ranging wild populations. For the first time, we propose to contribute to this question through dental microwear texture analysis (DMTA) using confocal microscopy. Characterising how the dietary behaviour of extant wild and domestic goats is reflected in DMTA is critical. For this purpose, we constructed a reference collection composed of three wild goat species (19 Capra pyrenaica from the southern Iberian Peninsula , 12 Capra ibex from the Alps and 12 Capra nubiana from the Near East) and 15 domestic goats (Capra hircus) reared in an extensive system in Mediterranean scrubland areas. The preliminary results indicate that DMTA allows discrimination of the groups of extant goats. The results obtained were used to characterise the palaeodiet of 16 goats (Capra format aegagrus) from two key Early and Middle Pre-Pottery Neolithic B sites in the southern Levant: Kharaysin (Zarqa, Jordan, ca. 8800-7500 cal BC) and Qarassa (As-Suweyda, Syria, 8700-8200 cal BCE). The higher variability in archaeological goats could be an indicator of controlled feedings. It could also support the hypothesis about diverse and experimental management systems at the first steps of animal domestication. This paper shows the potential of this tool to address the process of early animal domestication. Following this initial work, the reference collection will include a broader spectrum of livestock management strategies and the archaeological corpus will be expanded through new samples during upcoming excavations and the integration of additional sites. • The southern Levant could have been another primary centre of goat domestication. • The morphological distinction between wild and domestic goats of Pre-Pottery Neolithic is not a straightforward task. • Palaeodiet reconstruction using dental microwear texture can shed new light since diet of managed goats was controlled by their owners. • Dental microwear texture differs between extant domestic and wild goats. • Microwear variability of goats from archaeological sites is higher than that of extant goats, which could indicate controlled feedings. [ABSTRACT FROM AUTHOR]