1. Bifunctional silver-metal organic gels with catalytic and electrochemiluminescence properties applied for ratio detection of I27L gene.
- Author
-
Dai, Wenjie, Chen, Gaoxu, Sun, Shiyi, Zhen, Shujun, Huang, Chengzhi, Zhan, Lei, and Li, Yuanfang
- Subjects
- *
MATURITY onset diabetes of the young , *FLUORESCENCE resonance energy transfer , *BIFUNCTIONAL catalysis , *GENETIC variation , *CHARGE exchange , *AMPLIFICATION reactions - Abstract
• The bifunctional Ag MOGs were synthesized by a very simple method. • The Ag MOGs exhibited excellent AIECL performance. • The Ag MOGs enhanced the ECL signal of K 2 S 2 O 8 -O 2. • Combining Ag MOGs/K 2 S 2 O 8 -O 2 and DNA walker cycle amplification, an ultrasensitive ratio ECL-RET biosensor was constructed for I27L gene detection. The rate of maturity-onset diabetes of the young (MODY) is significantly increased by the common I27L gene variant of the hepatocyte nuclear factor-1α (HNF1A), hence achieving sensitive and precise I27L gene detection is the focus of early monitoring and diagnosis of HNF1A-MODY. In this study, we developed a silver-metal organic gels (Ag MOGs)/ K 2 S 2 O 8 -O 2 system-based electrochemiluminescence resonance energy transfer (ECL-RET) ratio biosensor for detecting the I27L gene. The novel Ag MOGs were synthesized by simply mixing Ag+ and Luminol at ambient temperature. On the one hand, Ag MOGs could generate excellent anodic ECL signals in phosphate buffer without requiring exogenous co-reactants, which were derived from the Luminol ligand. On the other hand, Ag MOGs facilitated the electrocatalytic reduction of K 2 S 2 O 8 by providing stable catalytic active sites and enhancing electron transfer rates, thereby leading to enhanced cathodic ECL emission from K 2 S 2 O 8 -O 2. We constructed an ECL-RET ratio biosensor utilizing a DNA walker cycle amplification strategy. The biosensor leveraged the anodic ECL emission from the Ag MOGs/K 2 S 2 O 8 -O 2 system as a donor and Atto 425 as a receptor, achieving ultra-sensitive detection of the I27L gene within a linear range of 100 amol/L to 100 pmol/L, and featuring a detection limit of 72 amol/L. Therefore, a direct synthesis technique of bifunctional materials with catalysis and electrochemiluminescence has been developed. Additionally, the ECL-RET ratio biosensor provided a universal platform for the detection of diabetes subtypes. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF