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Cyclists are exposed to hand--arm vibration (HAV) for prolonged periods of time during training sessions and competitions. The vibration can reduce perceived comfort, thus limiting the ability of the cyclist to control the bike in endurance sessions. The study of HAV in cyclists in a controlled environment allows for comparisons between the effects of different postures, materials and technical solutions on perceived discomfort and on the vibration transmitted to specific body segments. This paper describes the experimental setup, the measurement chain and the data processing for the evaluation of bike comfort in the laboratory. The setup is based on single-axis or multiaxial shakers; the time history of the input vibration can be derived from on-field measurements for comparative analyses or can be selected from among classical stimuli for frequency response function evaluation (sine sweep or white noise). Comfort can be quantified via questionnaires; objective measurements can be derived from vibrations measured at different body locations using wearable accelerometers or laser doppler vibrometers. A case study is presented and discussed. [ABSTRACT FROM AUTHOR]