7 results on '"Wang, Jin‐Lei"'
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2. Corrigendum to "Chitosan/poly (vinyl pyrollidone) coatings improve the antibacterial properties of poly(ethylene terephthalate)" [Appl. Surf. Sci. 258(20) (2012) 7801–7808].
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Wang, Bai-liang, Wang, Jin-lei, Li, Dan-dan, Ren, Ke-feng, and Ji, Jian
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CHITOSAN , *ETHYLENE , *SURFACE coatings - Published
- 2022
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3. Trx4, a novel thioredoxin protein, is important for Toxoplasma gondii fitness.
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Zhang, Zhi-Wei, Wang, Meng, Sun, Li-Xiu, Elsheikha, Hany M., Lei, Cheng-Lin, Wang, Jin-Lei, Fu, Bao-Quan, Luo, Jian-Xun, Zhu, Xing-Quan, and Li, Ting-Ting
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THIOREDOXIN , *LYTIC cycle , *GENE knockout , *GENOME editing , *PROTEINS , *TOXOPLASMA gondii - Abstract
Background: To successfully replicate within the host cell, Toxoplasma gondii employs several mechanisms to overcome the host cell defenses and mitigate the harmful effects of the free radicals resulting from its own metabolic processes using effectors such as thioredoxin proteins. In this study, we characterize the location and functions of a newly identified thioredoxin in T. gondii, which was named Trx4. Methods: We characterized the functional role of Trx4 in T. gondii Type I RH and Type II Pru strains by gene knockout and studied its subcellular localization by endogenous protein HA tagging using CRISPR-Cas9 gene editing. The enzyme-catalyzed proximity labeling technique, the TurboID system, was employed to identify the proteins in proximity to Trx4. Results: Trx4 was identified as a dense granule protein of T. gondii predominantly expressed in the parasitophorous vacuole (PV) and was partially co-localized with GRA1 and GRA5. Functional analysis showed that deletion of trx4 markedly influenced the parasite lytic cycle, resulting in impaired host cell invasion capacity in both RH and Pru strains. Mutation of Trx domains in Trx4 in RH strain revealed that two Trx domains were important for the parasite invasion. By utilizing the TurboID system to biotinylate proteins in proximity to Trx4, we identified a substantial number of proteins, some of which are novel, and others are previously characterized, predominantly distributed in the dense granules. In addition, we uncovered three novel proteins co-localized with Trx4. Intriguingly, deletion of trx4 did not affect the localization of these three proteins. Finally, a virulence assay demonstrated that knockout of trx4 resulted in a significant attenuation of virulence and a significant reduction in brain cyst loads in mice. Conclusions: Trx4 plays an important role in T. gondii invasion and virulence in Type I RH strain and Type II Pru strain. Combining the TurboID system with CRISPR-Cas9 technique revealed many PV-localized proximity proteins associated with Trx4. These findings suggest a versatile role of Trx4 in mediating the processes that occur in this distinctive intracellular membrane-bound vacuolar compartment. [ABSTRACT FROM AUTHOR]
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- 2024
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4. A newly characterized dense granule protein (GRA76) is important for the growth and virulence of Toxoplasma gondii.
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Zheng, Xiao-Nan, Sun, Li-Xiu, Elsheikha, Hany M., Li, Ting-Ting, Gao, Jin, Wu, Xiao-Jing, Zhang, Zhi-Wei, Wang, Meng, Fu, Bao-Quan, Zhu, Xing-Quan, and Wang, Jin-Lei
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GENE expression , *TOXOPLASMA gondii , *RNA sequencing , *PROTEINS , *DELETION mutation , *CRISPRS - Abstract
[Display omitted] • Dense granular protein GRA76 was more highly expressed in tachyzoites than in bradyzoites of Toxoplasma gondii. • GRA76 is important for the growth and virulence of T. gondii. • Deletion of gra76 in the Pru strain (PruΔ gra76) increased expression of bradyzoite-associated genes. • PruΔ gra76 exhibited increasd propensity for forming bradyzoites in vitro. Pathogenicity of the zoonotic pathogen Toxoplasma gondii largely depends on the secretion of effector proteins into the extracellular milieu and host cell cytosol, including the dense granule proteins (GRAs). The protein-encoding gene TGME49_299780 was previously identified as a contributor to parasite fitness. However, its involvement in parasite growth, virulence and infectivity in vitro and in vivo remains unknown. Here, we comprehensively examined the role of this new protein, termed GRA76, in parasite pathogenicity. Subcellular localization revealed high expression of GRA76 in tachyzoites inside the parasitophorous vacuole (PV). However, its expression was significantly decreased in bradyzoites. A CRISPR-Cas9 approach was used to knock out the gra76 gene in the T. gondii type I RH strain and type II Pru strain. The in vitro plaque assays and intracellular replication showed the involvement of GRA76 in replication of RH and Pru strains. Deletion of the gra76 gene significantly decreased parasite virulence, and reduced the brain cyst burden in mice. Using RNA sequencing, we detected a significant increase in the expression of bradyzoite-associated genes such as BAG1 and LDH2 in the PruΔ gra76 strain compared with the wild-type Pru strain. Using an in vitro bradyzoite differentiation assay, we showed that loss of GRA76 significantly increased the propensity for parasites to form bradyzoites. Immunization with PruΔ gra76 conferred partial protection against acute and chronic infection in mice. These findings show the important role of GRA76 in the pathogenesis of T. gondii and highlight the potential of PruΔ gra76 as a candidate for a live-attenuated vaccine. [ABSTRACT FROM AUTHOR]
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- 2024
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5. The Toxoplasma protein phosphatase 6 catalytic subunit (TgPP6C) is essential for cell cycle progression and virulence.
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Liang, Qin-Li, Nie, Lan-Bi, Elsheikha, Hany M., Li, Ting-Ting, Sun, Li-Xiu, Zhang, Zhi-Wei, Wang, Meng, Fu, Bao-Quan, Zhu, Xing-Quan, and Wang, Jin-Lei
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PHOSPHOPROTEIN phosphatases , *CELL cycle regulation , *CELL cycle , *TOXOPLASMA , *LYTIC cycle - Abstract
Protein phosphatases are post-translational regulators of Toxoplasma gondii proliferation, tachyzoite-bradyzoite differentiation and pathogenesis. Here, we identify the putative protein phosphatase 6 (TgPP6) subunits of T. gondii and elucidate their role in the parasite lytic cycle. The putative catalytic subunit TgPP6C and regulatory subunit TgPP6R likely form a complex whereas the predicted structural subunit TgPP6S, with low homology to the human PP6 structural subunit, does not coassemble with TgPP6C and TgPP6R. Functional studies showed that TgPP6C and TgPP6R are essential for parasite growth and replication. The ablation of TgPP6C significantly reduced the synchronous division of the parasite's daughter cells during endodyogeny, resulting in disordered rosettes. Moreover, the six conserved motifs of TgPP6C were required for efficient endodyogeny. Phosphoproteomic analysis revealed that ablation of TgPP6C predominately altered the phosphorylation status of proteins involved in the regulation of the parasite cell cycle. Deletion of TgPP6C significantly attenuated the parasite virulence in mice. Immunization of mice with TgPP6C-deficient type I RH strain induced protective immunity against challenge with a lethal dose of RH or PYS tachyzoites and Pru cysts. Taken together, the results show that TgPP6C contributes to the cell division, replication and pathogenicity in T. gondii. Author summary: Toxoplasma gondii is a protozoan parasite characterized by a highly spatially and temporally coordinated replication process. Some protein phosphatases are conserved among apicomplexan protozoa and regulate numerous cellular and biological processes, such as parasite proliferation, tachyzoite-bradyzoite differentiation and virulence. Here, we identify the role of the putative protein phosphatase 6 of T. gondii (TgPP6), and found two of its subunits, TgPP6C and TgPP6R, to be vital for the parasite replication and virulence. RHΔpp6c and RHΔpp6r exhibited various degrees of asynchronous division and morphological deformation during endodyogeny compared to wild-type parasites. TgPP6C affected the phosphorylation status of proteins involved in the parasite cell division. We also demonstrated the protective efficacy of immunization of mice using RHΔpp6c against acute and chronic infection by wild-type RH, PYS and Pru strains, respectively. These findings reveal novel roles of TgPP6C in the replication and virulence of T. gondii. [ABSTRACT FROM AUTHOR]
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- 2023
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6. The antioxidant protein glutaredoxin 1 is essential for oxidative stress response and pathogenicity of Toxoplasma gondii.
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Li, Ting‐Ting, Zhao, Dan‐Yu, Liang, Qin‐Li, Elsheikha, Hany M., Wang, Meng, Sun, Li‐Xiu, Zhang, Zhi‐Wei, Chen, Xiao‐Qing, Zhu, Xing‐Quan, and Wang, Jin‐Lei
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Glutaredoxins (Grxs) are ubiquitous antioxidant proteins involved in many molecular processes to protect cells against oxidative damage. Here, we study the roles of Grxs in the pathogenicity of Toxoplasma gondii. We show that Grxs are localized in the mitochondria (Grx1), cytoplasm (Grx2), and apicoplast (Grx3, Grx4), while Grx5 had an undetectable level of expression. We generated Δgrx1‐5 mutants of T. gondii type I RH and type II Pru strains using CRISPR‐Cas9 system. No significant differences in the infectivity were detected between four Δgrx (grx2‐grx5) strains and their respective wild‐type (WT) strains in vitro or in vivo. Additionally, no differences were detected in the production of reactive oxygen species, total antioxidant capacity, superoxide dismutase activity, and sensitivity to external oxidative stimuli. Interestingly, RHΔgrx1 or PruΔgrx1 exhibited significant differences in all the investigated aspects compared to the other grx2‐grx5 mutant and WT strains. Transcriptome analysis suggests that deletion of grx1 altered the expression of genes involved in transport and metabolic pathways, signal transduction, translation, and obsolete oxidation–reduction process. The data support the conclusion that grx1 supports T. gondii resistance to oxidative killing and is essential for the parasite growth in cultured cells and pathogenicity in mice and that the active site CGFS motif was necessary for Grx1 activity. [ABSTRACT FROM AUTHOR]
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- 2023
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7. Effect of deleting four Toxoplasma gondii calcium-binding EGF domain-containing proteins on parasite replication and virulence.
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Wang, Xin-Cheng, Li, Ting-Ting, Elsheikha, Hany M., Zheng, Xiao-Nan, Zhao, Dan-Yu, Wang, Jin-Lei, Wang, Meng, and Zhu, Xing-Quan
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APICOMPLEXA , *TOXOPLASMA gondii , *EPIDERMAL growth factor , *LIFE cycles (Biology) , *PROTEINS , *PROTEIN kinases , *CALCIUM-binding proteins , *GENE expression profiling - Abstract
Several calcium-binding proteins including calcium-dependent protein kinases play important roles in several facets of the intracellular infection cycle of the apicomplexan protozoan parasite Toxoplasma gondii. However, the role of the calcium-binding epidermal growth factor (EGF) domain-containing proteins (CBDPs) remains poorly understood. In this study, we examined the functions of four CBDP genes in T. gondii RH strain of type I by generating knock-out strains using CRISPR-Cas9 system. We investigated the ability of mutant strains deficient in CBDP1, CBDP2, CBDP3, or CBDP4 to form plaques, replicate intracellularly, and egress from the host cells. The results showed that no definite differences between any of these four CBDP mutant strains and the wild-type strain in terms of their ability to form plaques, intracellular replication, and egress. Additionally, CBDP mutants did not exhibit any significant attenuated virulence compared to the wild-type strain in mice. The expression profiles of CBDP2-4 genes were conserved among T. gondii strains of different genotypes, life cycle stages, and developmental forms. Whether other CBDP genes play any roles in the pathogenicity of T. gondii strains of different genotypes remains to be elucidated. [ABSTRACT FROM AUTHOR]
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- 2023
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