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[Display omitted] • The Mn redox cycle removed 94.67 % of E2 within 12 h (0.079 mg L−1h−1). • Interaction of reactive oxygen species and Mn(III) accelerated E2 removal. • Bioreactor showed 89.63% of NO 3 −-N and 97.57% of 17β-estradiol removal efficiency. • Community assembly enhanced the metabolic and physiological activity of bioreactor. • Different elemental cycles in the community were coupled with contaminant removal. The application of bio-manganese (Mn) redox cycling for continuous removal of contaminants provides promise for addressing coexisting contaminants in groundwater, however, the feasibility of constructing Mn redox cycling system (MCS) through community assembly remains to be elucidated. In this study, Mn-reducing strain MFG10 and Mn-oxidizing strain MFQ7 synergistically removed 94.67 % of 17β-estradiol (E2) within 12 h. Analysis of potential variations in Mn oxides suggested that MCS accelerated the production of reactive oxygen species (ROS) and Mn(III), which interacted to promote E2 removal. After continuous operation of the Mn ore-based immobilized bioreactor for 270 days, the experimental group (EG) achieved average removal efficiencies of 89.63 % and 97.57 % for NO 3 −-N and E2, respectively. High-throughput sequencing results revealed complex symbiotic relationships in EG. Community assembly significantly enhanced the metabolic and physiological activity of the bioreactor, which promoting the expression of core functions including nitrogen metabolism, Mn cycling and organic matter resistance. [ABSTRACT FROM AUTHOR]