1. Effect of the photosynthesis inhibitors on hydrogen production by non-heterocyst cyanobacterial strains.
- Author
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Kamshybayeva, Gulzhanay K., Kossalbayev, Bekzhan D., Sadvakasova, Asemgul K., Bauenova, Meruyert O., Zayadan, Bolatkhan K., Krapivina, Anastasia A., Sainova, Gaukhar A., Alharby, Hesham F., and Allakhverdiev, Suleyman I.
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HYDROGEN production , *CYANOBACTERIAL toxins , *PHOTOSYNTHESIS , *HYDROGEN as fuel , *PHOTOSYSTEMS , *SYNECHOCYSTIS , *CYANOBACTERIA - Abstract
The energy of cyanobacterial hydrogen (H 2) produced via bio-photolysis is being investigated as a potential solution to early-century environmental challenges. The main limiting factors of cyanobacterial H 2 photoproduction are the availability of electrons for [NiFe]-hydrogenase (H 2 ase) and the suppression of bidirectional H 2 ase activity induced by O 2 acquired from water molecules splitting in photosystem II. The current study investigated how photosynthetic inhibitors (PIs) affected H 2 production in non-N 2 -fixing cyanobacteria. Study findings revealed a rather high H 2 yield in Synechocystis sp. PSU 1262, as well as a beneficial (14.2-fold) influence of 500 μmol KCN on the H 2 production by the aforesaid strain. A 12/6-h light/dark cycle increased H 2 production by 80.3% in cells supplemented with 500 μmol KCN. Under the optimised conditions, the photobiological H 2 production of Synechocystis sp. PSU 1262 increased from 49.6 to 1552 nmol H 2 mg−1 Chl a h−1. PIs suppressed chlorophyll a concentration under illumination, lowering the O 2 levels, which enhanced bidirectional H 2 ase activity in Synechocystis sp. PSU 1262 cells. Applying varied light modes, preceded by the incorporation of PIs at optimal concentrations in H 2 production by research cyanobacterial strains, improved the H 2 yield and contributed significantly to the research originality. [Display omitted] • Six species of cyanobacteria from different ecosystems were isolated and identified. • The H 2 -producing activity of the strains was studied in the dark and in the light. • The effect of four inhibitors of photosynthesis on H 2 production was studied. • The effect of light and dark modes was evaluated on H 2 production by cyanobacteria. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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