Peptide B, the N-terminal fragment of thioredoxin obtained by cyanogens bromide treatment was aminoethylated and digested with pepsin. Eight peptides were separated by high voltage paper electrophoresis and chromatography and characterized with respect to amino acid sequence. The results account for all 37 residues peptide B. The N-terminal sequence of peptide B was determined as: Ser-Asp-Lys-Ile-Ile-His-Leu-Thr-Asp-Asp-Ser-Phe. [ABSTRACT FROM AUTHOR]
Presents an investigation of the sequencing of 65 amino acid residues at the carboxyl-terminus. Use of methods such as enzymic or chemical cleavages of the peptide chain; Fractionation of peptides by Sephadex or Dowex column chromatography, paper elctrophoresis or chromatography.
*CHLOROPHYLL, *SPECTROPHOTOMETRY, *ANALYTICAL chemistry, *SPECTROPHOTOMETERS, *PLANT physiology, *PLANT development
Abstract
The various methods for chlorophyll determination, found in the literature may give chlorophyll values differing by as much as 20 &percent;. Not only the choice of solvent and equations but also the routines and conditions for proceeding of samples can be large sources of errors. In the present paper some recommendations are given concerning sampling and storage of plant material, extraction and determination of chlorophylls. A simple and fast method for chlorophyll determinations is described and recommended as a suitable method for most ecological and eco-physiological investigations in which chlorophylls are determined. The principle of the method is that the total amount of chlorophylls is determined in a colorimeter calibrated for chlorophyll measurements. Results form a prototype of "Cholorphyllometer" are presented and compared with spectrophotometric determinations. [ABSTRACT FROM AUTHOR]