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The vegetation coverage of temperate grasslands in China has changed substantially due to climate change during the past decades, which significantly affects the function of grassland ecosystems. To appropriately carry out adaptive management and protect temperate grassland vegetation, it is important to understand the variations in fractional vegetation coverage (FVC) of China's temperate grasslands and how they are responding to climate change. Using the GIMMS NDVI and climatic datasets, this study explored the dynamics of FVC and their climatic drivers across the temperate grassland region of China during 1982∼2015. The results showed that the growing season mean FVC increased by 0.12% per year during 1982∼2015. The increases in precipitation and minimum temperature in the growing-season (especially in spring) could enhance the FVC of various vegetation types. In summer, the FVC of temperate steppe and desert steppe could drastically increase with increasing precipitation. In addition, this study found that the impacts of daytime and night-time warming on the FVC of temperate grasslands were asymmetric. Daytime warming can moderately increase FVC of temperate grasslands, while night-time warming could significantly increase it. Furthermore, the increase in summer daytime and night-time temperatures leads to a weak decrease and a moderate increase in FVC, respectively. This asymmetric effect was more evident for the temperate steppe and desert steppe in the central area. In autumn, the temperatures increase had significant positive impacts on the FVC of temperate meadows and steppes. This study highlights the differences in the impacts of climate change at different time scales on the FVC of grasslands with various vegetation types, and indicates that the asymmetric influences of daytime and night-time temperatures in different seasons on FVC must be included in calculating the vegetation coverage of China's temperate grasslands. The results could provide information for maintaining grassland ecosystem functions and managing environmental systems. • The FVC of temperate grasslands has increased by 0.12% per year from 1982 to 2015. • Increases in growing season precipitation and minimum temperature enhanced the FVC. • Nighttime and daytime temperatures have asymmetric effects on the FVC. [ABSTRACT FROM AUTHOR]

To evade host antiviral response, viruses have evolved to take advantage of their noncoding RNAs (ncRNAs). Snakehead vesiculovirus (SHVV), a newly isolated fish rhabdovirus from diseased hybrid snakehead, has caused high mortality to the cultured snakehead fish during the past years in China. However, little is known about the mechanisms of its pathogenicity. Our study revealed that overexpression of the 30-nt leader RNA promoted SHVV replication. RNA-protein binding investigation revealed that SHVV leader RNA could interact with host 40S ribosomal protein S8 (RPS8) and 60S ribosomal protein L13a (L13a). Furthermore, we found that SHVV infection upregulated RPS8 and L13a, and in turn, overexpression of RPS8 or L13a inhibited, while knockdown of RPS8 or L13a promoted, SHVV replication, suggesting that RPS8 and L13a acted as host antiviral factors in response to SHVV infection. In addition, our study revealed that RPS8- or L13a-mediated inhibition of SHVV replication could be restored by co-transfection with leader RNA, suggesting that the interaction between leader RNA and RPS8 or L13a might affect the anti-SHVV effects of RPS8 and L13a. Taken together, these results suggest that SHVV leader RNA can interact with the host antiviral factors RPS8 and L13a, and promote SHVV replication. This study provides a better understanding of the molecular mechanism of the pathogenesis of SHVV and a potential antiviral strategy against SHVV infection. • SHVV leader RNA could interact with the host antiviral factors RPS8 and L13a. • Leader RNA can restore L13a- or RPS8-mediated inhibition of SHVV replication. [ABSTRACT FROM AUTHOR]