1. Preparation of poly(caffeic acid)-coated epitope molecularly imprinted polymers and investigation of adsorption performance toward ovalbumin.
- Author
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Zhu, Ran, Wang, Xindi, Ma, Jiutong, and Jia, Qiong
- Subjects
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IMPRINTED polymers , *CAFFEIC acid , *FOOD allergy , *ADSORPTION capacity , *ADSORPTION (Chemistry) , *ALLERGIES - Abstract
• Epitope molecularly imprinted polymer was prepared by autoxidation of caffeic acid. • High adsorption capacity and selectivity toward ovalbumin were achieved. • The polymer was successfully applied to enrich ovalbumin in egg white samples. Food allergy can lead to severe allergic reactions that are potentially fatal for human, hence the detection of food allergens such as ovalbumin (OVA) is important. In this study, a poly(caffeic acid)-coated epitope molecularly imprinted polymer (EMIP) was prepared by chelation and autoxidation of caffeic acid with hexamethylenediamine. EMIP has not only imprinted cavities highly matched with OVA in size and spatial structure, but also externally abundant hydrophilic groups, resulting in few non-specific binding and good hydrophilicity. With high specificity, significant paramagnetism, and great reusability, EMIP can distinguish OVA from other proteins and selectively enrich OVA in egg white samples, which opens up a promising route to the determination of allergens in food products. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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