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Simple Summary: The aim of our study was to investigate the effects of dietary supplementation with different protein levels after grazing on growth performance as well as on the proteomics of the LL muscle of Jersey-yak. The results showed that a certain amount of crude protein supplementation to Jersey-yak at the end of grazing significantly improved growth performance and increased their economic efficiency. In addition, we analyzed the proteomic differences in the longest dorsal muscle of Jersey-yak. From our analyses, we identified differences in the abundance of 434 proteins involved in pathways related to muscle growth and development and energy metabolism, among others. Several of the proteins enriched in these pathways, such as MYH8, are associated with myoblast development and differentiation as well as muscle development. In conclusion, the results of this study provide proteomic insights into the different feeding patterns of yak crossbred progeny, suggesting that a greater economic income can be achieved by improving their feeding conditions. Jersey-yak is a hybrid offspring of Jersey cattle and yak (Bos grunniens). Changing the feeding system of Jersey-yak can significantly improve its growth performance. In this study, tandem mass tag (TMT) proteomics technology was used to determine the differentially expressed proteins (DEPs) of the longissimus lumborum (LL) muscle of Jersey-yak fed different protein levels of diet. The results showed that compared with the traditional grazing feeding, the growth performance of Jersey-yaks was significantly improved by crude protein supplementation after grazing. A total of 3368 proteins were detected in these muscle samples, of which 3365 were quantified. A total of 434 DEPs were identified. Through analyses, it was found that some pathways related to muscle growth and development were significantly enriched, such as Rap1 signaling pathway, mTOR signaling pathway, and TGF-beta signaling pathway. A number of DEPs enriched in these pathways are related to muscle cell development, differentiation, and muscle development, including integrin subunit alpha 7 (ITGA7), myosin heavy chain 8 (MYH8), and collagen type XII alpha 1 chain (COL12A1). In conclusion, the results of this study provide insights into the proteomics of different feeding patterns of Jersey-yak, providing a stronger basis for further understanding the biological mechanism of hybrid varieties. [ABSTRACT FROM AUTHOR]

As the rapid and accurate screening of infectious diseases can provide meaningful information for outbreak prevention and control, as well as owing to the existing limitations of the polymerase chain reaction (PCR), it is imperative to have new and validated detection techniques for SARS-CoV-2. Therefore, the rationale for outlining the techniques used to detect SARS-CoV-2 proteins and performing a comprehensive comparison to serve as a practical benchmark for future identification of similar viral proteins is clear. This review highlights the urgent need to strengthen pandemic preparedness by emphasizing the importance of integrated measures. These include improved tools for pathogen characterization, optimized societal precautions, the establishment of early warning systems, and the deployment of highly sensitive diagnostics for effective surveillance, triage, and resource management. Additionally, with an improved understanding of the virus' protein structure, considerable advances in targeted detection, treatment, and prevention strategies are expected to greatly improve our ability to respond to future outbreaks. [Display omitted] • This review features meaningful information for preventing and controlling outbreaks. • This is a clear summary of the structure and function of SARS-CoV-2 proteins. • A comprehensive comparison of relevant SARS-CoV-2 protein assays is revealed. • This review aims to provide a future reference for detecting similar viral proteins. [ABSTRACT FROM AUTHOR]

As the rapid and accurate screening of infectious diseases can provide meaningful information for outbreak prevention and control, as well as owing to the existing limitations of the polymerase chain reaction (PCR), it is imperative to have new and validated detection techniques for SARS-CoV-2. Therefore, the rationale for outlining the techniques used to detect SARS-CoV-2 proteins and performing a comprehensive comparison to serve as a practical benchmark for future identification of similar viral proteins is clear. This review highlights the urgent need to strengthen pandemic preparedness by emphasizing the importance of integrated measures. These include improved tools for pathogen characterization, optimized societal precautions, the establishment of early warning systems, and the deployment of highly sensitive diagnostics for effective surveillance, triage, and resource management. Additionally, with an improved understanding of the virus' protein structure, considerable advances in targeted detection, treatment, and prevention strategies are expected to greatly improve our ability to respond to future outbreaks. [Display omitted] • This review features meaningful information for preventing and controlling outbreaks. • This is a clear summary of the structure and function of SARS-CoV-2 proteins. • A comprehensive comparison of relevant SARS-CoV-2 protein assays is revealed. • This review aims to provide a future reference for detecting similar viral proteins. [ABSTRACT FROM AUTHOR]