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The model HYDRUS-1D was used to simulate soil water dynamics of full and deficit irrigated maize grown under a rainout shelter during two crop seasons. Four irrigation treatments were established based on the amount of water applied to fulfil crop water requirements. Treatment D1 was irrigated to fully satisfy crop water requirements, while treatments D2 (mild deficit), D3 (moderate deficit), and D4 (severe deficit) were for increased controlled water stress conditions. The computation and partitioning of evapotranspiration data into soil evaporation and crop transpiration was carried out with the SIMDualKc model, and then used with HYDRUS-1D. The soil hydraulic properties were determined from numerical inversion of field water content data. The compensated root water uptake mechanism was used to describe water removal by plants. The HYDRUS-1D model successfully simulated the temporal variability of soil water dynamics in treatments irrigated with full and deficit irrigation, producing RMSE values that varied between 0.014 and 0.025 cm 3 cm −3 when comparing model simulations with field measurements. Actual transpiration varied between 224 and 483 mm. Potential transpiration reductions varied from 0.4 to 48.8% due to water stress, but plants were able to compensate for the water deficits in the surface layers by removing more water from the deeper, less stressed layers. HYDRUS-1D water balance estimates were also comparable with the corresponding ones determined with the SIMDualKc water balance model. Both modelling approaches should contribute to improve the web-based IRRIGA system, used to support farm irrigation scheduling in Brazil. [ABSTRACT FROM AUTHOR]

In a recent report, we showed that it is possible to establish the culture of Human Corneal Endothelial Cells (HCEnCs) from older donor corneas (usually over 65 year olds) when left to attach in the presence of a viscoelastic solution, potentially increasing the donor pool for culturing HCEnCs. Therefore, we set out to evaluate the outcome of using a viscoelastic solution (Viscoat) to accelerate the attachment of passaged cultured human corneal endothelial cells (HCEnCs). The cells from 28 donor tissues were isolated using peel-and-digest method and evenly seeded into two wells of an 8-well chamber slide. The cells were left to attach after topical application of Viscoat. At confluence, one well was subjected to end-stage characterization, whereas the other well was passaged into another two wells. The cells at P1 were attached with and without the use of Viscoat. The growth rate was monitored; and at confluence, morphometric analysis, corneal endothelial specific (CD166-Tag1A3 & PRDX6-Tag2A12), mitochondrial and respiration assessment (Tom-20 and Seahorse); function-associated (Na+/K+ATPase & ZO-1); proliferative (Ki-67) marker analysis, and viability (Hoechst, Ethidium Homodimer and Calcein AM-HEC) studies were performed. Cells at P0 (with Viscoat) showed 100% confluence at day 9. Cells at P1 with and without Viscoat showed significant difference of confluence 67.0% v 18.8% respectively (p < 0.05). Confluence rate, cell density, hexagonality, Ki-67 positivity and mitochondrial intensity was significantly higher (p < 0.05), whereas cell-area and polymorphism was significantly lower (p < 0.05) in the cells attached with Viscoat compared with the cells attached without Viscoat. There was no significant difference in oxygen consumption rate between the groups. In conclusion, we observed that acceleration in the attachment of passaged HCEnCs with the assistance of Viscoat, could be beneficial for the propagation of HCEnCs isolated from older donors, to increase their propensity to proliferate, without loss of the expression of vital proteins and heterogeneity in cellular morphology. • Attachment of the Human Corneal Endothelial Cells (HCEnCs) can be accelerated using a viscoelastic (Viscoat) solution. • HCEnCs passaged with Viscoat helps in cellular attachment and proliferation. • Passaged HCEnCs with Viscoat maintain the expression of vital proteins and cellular morphology. [ABSTRACT FROM AUTHOR]