1. CKAMP44: A Brain-Specific Protein Attenuating Short-Term Synaptic Plasticity in the Dentate Gyrus.
- Author
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von Engelhardt, Jakob, Mack, Volker, Sprengel, Rolf, Kavenstock, Netta, Ka Wan Li, Stern-Bach, Yael, Smit, August B., Seeburg, Peter H., and Monyer, Hannah
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BRAIN chemistry , *NEUROPLASTICITY , *DENTATE gyrus , *BRAIN physiology , *PROTEIN research , *NEUROPHYSIOLOGY , *NEURAL receptors , *SYNAPSES , *GENE expression , *GENETIC regulation , *PHYSIOLOGY - Abstract
CKAAAP44, identified here by a proteomic approach, is a brain-specific type I transmembrane protein that associates with AAAPA receptors in synaptic spines. CKAAAP44 expressed in Xenopus oocytes reduced GluA1- and A2-mediated steady-state currents, but did not affect kainate- or N-methyl-D-aspartate (NMDA) receptor-mediated currents. Mouse hippocampal CA1 pyramidal neurons expressed CKAMP44 at low abundance, and overexpression of CKAMP44 led to stronger and faster AMPA receptor desensitization, slower recovery from desensitization, and a reduction in the paired-pulse ratio of AMPA currents. By contrast, dentate gyrus granule cells exhibited strong CKAMP44 expression, and CKAMP44 knockout increased the paired-pulse ratio of AMPA currents in lateral and medial perforant path-granule cell synapses. CKAMP44 thus modulates short-term plasticity at specific excitatory synapses. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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