1. Puerarin Inhibits oxLDL-Induced Macrophage Activation and Foam Cell Formation in Human THP1 Macrophage.
- Author
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Heng Zhang, Zhenhua Zhai, Hongyu Zhou, Yao Li, Xiaojie Li, Yuhan Lin, Weihong Li, Yueping Shi, and Ming-Sheng Zhou
- Subjects
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ATHEROSCLEROSIS prevention , *CARDIOVASCULAR disease prevention , *LOW density lipoproteins , *APOPTOSIS , *ATHEROSCLEROSIS , *BIOCHEMISTRY , *CARDIOVASCULAR diseases , *CELL physiology , *CELL receptors , *CELLULAR signal transduction , *CYTOKINES , *FLOW cytometry , *GENE expression , *INFLAMMATORY mediators , *INTERLEUKINS , *MACROPHAGES , *PHENOMENOLOGY , *TUMOR necrosis factors , *DNA-binding proteins , *ISOFLAVONES , *TOLL-like receptors , *IN vitro studies , *THERAPEUTICS - Abstract
Puerarin, an isoflavone derived from Kudzu roots, has been widely used for treatment of cardiovascular and cerebral vascular diseases in China and other Asian countries. However, the underlying mechanisms are largely unknown. The present study investigated whether puerarin inhibited atherogenic lipid oxLDL-mediated macrophage activation and foam cell formation in human THP1 macrophage. Treatment with oxLDL significantly increased the mRNA expression of proinflammatory cytokines tumor necrosis factor α (TNFα, 160%) and interleukin (IL) 1β (13 fold) accompanied by upregulation of toll-like receptor 4 (TLR4, 165%) and the ratio of phospho-IκBα/IκBα in THP1 macrophage. Puerarin dose-dependently prevented an increase in oxLDL-induced proinflammatory gene expression with downregulation of TLR4 and the ratio of phospho-IκBα/IκBα. Furthermore, puerarin prevented oxLDL-mediated lipid deposition and foam cell formation associated with downregulation of scavenger receptor CD36. Flow cytometry analysis showed that puerarin reduced the number of early apoptotic cells of macrophages induced by oxLDL. Our results show that puerarin has anti-inflammatory and antiatherogenic effects in vitro; the underlying mechanisms may involve the inhibition of TLR4/NFκB pathway and downregulation of CD36 expression. The results from the present study provide scientific evidence and may expand our armamentarium to use puerarin for prevention and treatment of cardiovascular and atherosclerotic diseases. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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