1. Antibacterial Activity and Mechanisms of Essential Oil from Citrus medica L. var. sarcodactylis.
- Author
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Li, Ze-Hua, Cai, Ming, Liu, Yuan-Shuai, Sun, Pei-Long, Luo, Shao-Lei, and Mancianti, Francesca
- Subjects
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ESSENTIAL oils , *CITRON , *ANTIBACTERIAL agents , *FOODBORNE diseases , *STAPHYLOCOCCUS aureus - Abstract
In this work, antibacterial activity of finger citron essential oil (FCEO, Citrus medica L. var. sarcodactylis) and its mechanism against food-borne bacteria were evaluated. A total of 28 components in the oil were identified by gas chromatography-mass spectrometry, in which limonene (45.36%), γ-terpinene (21.23%), and dodecanoic acid (7.52%) were three main components. For in vitro antibacterial tests, FCEO exhibited moderately antibacterial activity against common food-borne bacteria: Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Micrococcus luteus. It showed a better bactericidal effect on Gram-positive bacteria than Gram-negative. Mechanisms of the antibacterial action were investigated by observing changes of bacteria morphology according to scanning electron microscopy, time-kill analysis, and permeability of cell and membrane integrity. Morphology of tested bacteria was changed and damaged more seriously with increased concentration and exposure time of FCEO. FCEO showed a significant reduction effect on the growth rate of surviving bacteria and lead to lysis of the cell wall, intracellular ingredient leakage, and consequently, cell death. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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