Search

Dry direct-seeded rice (dry-DSR) is an efficient, resource-saving and environmentally friendly cropping system. The employment of water-saving and drought-resistant rice (WDR) for dry direct-seeding can better meet the needs of dry-direct seeding systems. However, the decline in seedling emergence rate and poor seedling growth are the main bottlenecks under current direct-seeded rice production. Seed treatment is a sustainable and effective technique to overcome these issues. Therefore, growth chamber and field experiments were conducted to assess the impact of poplar wood vinegar (WV) priming and rice straw biochar (BC) coating on emergence, establishment, growth, physio-biochemical events, and ultimate yield. We treated the seeds of WDR viz., Hanyou 73 with WV, BC, and co-treatment WV + BC. The results showed that seed priming with 1:50 WV concentration and coating with 20% BC content was the optimal ratio for promoting germination and seedling growth. The field evaluation indicated that individual WV and BC markedly promoted the final emergence by 58% and 31%, respectively, while co-treatment WV + BC increased by 67%. Likewise, WV and BC significantly enhanced total seedling biomass by 26% and 10%, respectively, and the respective enhancement of WV + BC was 31%. For ultimate yield, WV and BC produced 12% and 19% higher grain yield, respectively, whereas WV + BC yielded 20%. The above results revealed that WV and WV + BC were the most effective treatment. Our findings may provide new avenues for advancing pre-sowing seed treatments facilitating the stand establishment and grain yield of dry direct-seeded rice. [ABSTRACT FROM AUTHOR]

Compensating nitrogen input and increasing nitrogen utilization efficiency (NUtE) are necessary for sustainable crop production. Research studies on the co-application of biochar and nitrogen to promote rapeseed growth, soil fertility, and improve nutrient utilization efficiency are found to be limited. This study aims to examine the integral effects of biochar and nitrogen over soil fertility, rapeseed growth and nitrogen utilization efficiency. The experiment was conducted in a completely randomized design to assess various morphological, physiological and biochemical traits of rapeseed and soil chemical properties under the application of four nitrogen levels (0, 75, 225, and 450 kg ha−1 equivalent to 0, 133, 400 and 800 mg nitrogen pot−1) and four biochar levels (0, 5, 10, and 15 MT ha−1 equivalent to 0, 10, 20 and 30 g biochar pot−1 soil by weight basis). The results of this study showed that the integral application of biochar at 30 g pot−1 and nitrogen at 800 mg pot−1 at 45 days after sowing (DAS) and 60 days after sowing (DAS) compensated the amount of nitrogen and increased soil organic carbon (SOC 69%), total nitrogen accumulation (TNA) (93% and 62%), leaf biomass (60% and 33%), stem biomass (20% and 22%) and photosynthesis (20% and 17%) at 45 DAS and 60 DAS, respectively as compared to a single application of nitrogen. However, under the conditions of no nitrogen or 75 kg ha−1 nitrogen application, the application of biochar to promote the growth of rapeseed plants is limited due to the less nitrogen supply. In contrast, the application of nitrogen at 800 mg pot−1 and biochar at 10 or 20 g pot−1 showed that the growth of early seedlings was adversely affected due to high nitrogen concentration in soil. The findings of the present study imply that within the appropriate nitrogen application range, the interaction of biochar and nitrogen might have an economical approach towards better utilization of nitrogen and sustainable crop production. [ABSTRACT FROM AUTHOR]

An engineered cementitious composite (ECC) belongs to a type of high-performance fiber-reinforced materials. Fiber alignment causes the anisotropy of such materials. Herein, the influence of the fiber orientation on water and ion penetration into an ECC was studied. Fiber alignment was achieved using an extrusion approach. Water absorption, sorptivity, chloride penetration resistance, sulfate attack resistance, and freezing–thawing resistance of specimens with fiber aligned horizontally (AH), vertically (AV), and randomly (R), corresponding to the direction of the exposure surface that was studied. The results showed that fibers oriented perpendicular to the water path delayed water migration into the ECC matrix. The sorptivity was significantly affected by the fiber direction. The sorptivity of the AH specimens was 35% and 13% lower than that of the AV and R specimens, respectively. After 180 days of exposure, the chloride penetration depth of the AH specimens was 5.7 mm, which is 13.6% and 20.8% lower than that of the AV and R specimens, respectively. The sulfate ingress profile indicates that the fiber–matrix interface oriented perpendicular to the penetration path can effectively delay sulfate migration. The fiber orientation also influences the compressive strength gain under immersion conditions (Na2SO4 solution, Na2SO4 + NaCl solution, and water). Compared with the AH and R specimens, the AV specimens are more sensitive to the immersion condition. In contrast, the fiber orientation has no significant effect on ECC specimens under freeze–thaw cycles. These findings indicate that controlling the fiber alignment and orientation in an ECC can improve its durability under certain exposure conditions. [ABSTRACT FROM AUTHOR]

The genus Myotis is one of the most diverse and widely distributed mammals, providing a good model for studies of speciation and diversification across large geographic scales. However, the classification within this genus has long been chaotic. Taxonomic revisions based on multiple data sources are essential and urgent. In this study, morphometrics and genetic markers with different modes of inheritance were used to clarify the taxonomy of Myotis distributed in China. Based on 173 mitochondrial Cytb sequences and five morphological characteristics, 114 specimens collected nationwide over the past 20 years were assigned to 11 Myotis species. All Chinese samples classified into M. davidii and M. longipes were revised to M. alticraniatus and M. laniger. Then, two nuclear fragments (Rag2 and Chd1) and Cytb sequences from representative individuals of Chinese Myotis were used for multilocus phylogeny reconstruction and genetic divergence evaluation. The phylogenetic relationships were clearly demonstrated in the species tree: M. alticraniatus and M. laniger; M. fimbriatus, M. pilosus, M. macrodactylus, and M. petax; and M. pequinius, M. chinensis, and M. blythii formed three strongly supported monophyletic clades. Mitochondrial divergence was almost 10 times that of nuclear divergence, with interspecific K2P distances ranging from 8% to 20% for Cytb and 0.3% to 2.3% for concatenated nuclear genes. Low levels of genetic divergence were observed between M. alticraniatus and M. laniger, as well as M. fimbriatus and M. pilosus. These results provide new insights into the taxonomy and phylogeny of Myotis bats in China and are important for the future research and conservation of Chinese Myotis. [ABSTRACT FROM AUTHOR]

Freezing severely impacts potato production. Deciphering the pathways and metabolites that regulate the freezing tolerance of potato is useful in cultivation and breeding for hardiness. In the present study, Solanum acaule was identified to be more freezing tolerant than S. tuberosum. Furthermore, the two genotypes before/after exposure to 4 °C for 7 d with additional −1 °C for 12 h were analysed by RNA-seq and metabolomics, and the results were compared with the previous −1 °C for 12 h. The results showed that S. acaule activated numerous genes that differed from those of S. tuberosum. Among the genes, five pathways, such as the hormone signalling pathway, which includes salicylic acid, were enriched. Further metabolomics analysis showed that the content of salicylic acid was improved in S. acaule in response to −1 °C for 12 h. Moreover, exogenous application of 0.1 mM salicylic acid to potato was shown to improve constitutive freezing tolerance and increase the expression of HSFC1. Following transcriptome and metabolome analyses, it was documented that the content of SA that increased in freezing-tolerant S. acaule after exposure to cold condition, associated with the SA signalling pathway, enhanced potato freezing tolerance, probably through HSFC1. [ABSTRACT FROM AUTHOR]

Low temperature seriously affects the growth of crops and poses a huge threat to food production. The application of wood vinegar can be used as a cost-effective and environmentally friendly strategy to promote crop growth and enhance stress resistance, and the physiological resistance to low-temperature stress of rapeseed still needs further research. The present study investigated the effects of spraying wood vinegar on the growth, photosynthesis, osmotic adjustment, and antioxidant enzymes of rapeseed seedlings under low-temperature stress. The results showed that spraying wood vinegar at normal temperature reduced the stomatal conductance but increased the leaf area and total biomass of rapeseed and enhanced stomatal density and water use efficiency. The leaf area and total biomass of rapeseed sprayed with wood vinegar at low temperature improved by 22% and 31%, respectively, and stomatal density and water use efficiency increased by 14% and 83%, respectively, and intercellular CO2 concentration and stomatal conductance were reduced by 9% and 41%, compared to the low-temperature, respectively. Besides, the application of wood vinegar liquid improved the proline, soluble protein, and soluble sugar content of leaves by 208%, 38%, and 115%, respectively, and the activity of superoxide dismutase increased by 27%, the content of malondialdehyde decreased by 46%, compared to the low-temperature. Spraying wood vinegar could alleviate low-temperature stress by improving the anti-oxidant enzyme content and osmoprotectants, reducing the stomatal conductance, and enhancing water use efficiency. These results provide new insights for wood vinegar to relieve the low-temperature stress of rapeseed, and this strategy can be used for low-temperature rapeseed cultivation and management, and benefit farmers' plant profit. [ABSTRACT FROM AUTHOR]

Soil relative water content and seed plumpness have been shown to be the key factors affecting seed germination and seedling growth of rice under direct drought cropping. It remains to be determined whether seed germination and seedling growth of water-saving and drought-resistant rice (WDR) and conventional rice with the same proportion of rice seed have the same response to soil moisture changes. The purpose of this study was to investigate the seed germination and physiological characteristics of the rice cultivars Guangliangyou 1813 (GLY-1813,indica hybrid rice) and Hanyou 73 ((HY-73), WDR) with four different specific gravities (T1, T2, T3, and T4; the rice seeds were divided into four specific gravity levels by weight using saline water, the representative specific gravities were <1.0, 1.0–1.1, 1.1–1.2 and >1.2 kg m−3, respectively), at five soil moisture content gradients (soil relative water contents of 10–20%, 20–40%, 40–60%, 60–80%, and 80–100%), under dry direct seeding conditions. The results showed that GLY-1813 had a higher germination potential, germination and seedling emergence rates, greater root dry weight, seedling dry weight, root oxidation activity, and chlorophyll content, and lower malondialdehyde (MDA) content when the soil relative water content was 20–40% or 40–60%. Cultivar HY-73 had the highest germination rate and seedling physiological activity at 20–40% relative water content; its growth vigor was better than that of GLY-1813 at the same soil moisture level. In conclusion, the soil relative water content for seed germination of HY-73 was 20–40%, which was less than that of GLY-1813. When soil relative water content was sufficient for seed germination and growth, the higher the plumpness of the rice seed, the easier it was to resist the negative effects of an adverse growth environment. [ABSTRACT FROM AUTHOR]

Wood vinegar is formed by the condensation of smoke produced during the production of biochar. It mainly contains acetic acid, butyric acid, catechol, and phenol. Wood vinegar has a compound effect of promoting crop growth similar to plant growth regulators and is environmentally friendly. Moreover, it can enhance the biological and abiotic resistance of crops. In this study, foliar spraying was carried out systematically in the field with the hybrid variety of Huayouza 9 for two years to study the effects of wood vinegar and its compounds on the growth of rapeseed (Brassica napus L.). We applied four treatments with tap water as a control (CK), namely wood vinegar diluted 400-fold (M), M mixed with gibberellin (T1), M mixed with sodium D-gluconate (T2), and M mixed with melatonin (T3). They were sprayed in the seedling stage and overwintering stage, respectively. The results showed that the seed yield, the leaf area index, and the number of pods per plant of rapeseed treated with M increased by an average of 9.58%, 23.45%, and 23.80% in two years as compared to the CK, respectively. Compared with M, the seed yield of rapeseed treated with T1, T2, and T3 increased by an average of 7.88%, 6.90%, and 1.32% in two years, respectively. The treatments also improved the quality of rapeseed. In particular, the glucosinolate content of rapeseed treated with T2 and T3 decreased by an average of 12.83% and 6.72% in two years compared to the CK, respectively. The four treatments selected in the current study improved the resistance of rapeseed at the low temperature of 2–6 °C by increasing the activity of superoxide dismutase and proline and soluble protein contents, as compared to the CK. Besides, all treatments containing M reduced the incidence of Sclerotinia sclerotiorum and Peronospora parasitica (downy mildew) in rapeseed. More specifically, the T3 treatment significantly decreased the infection rate of these two diseases mentioned above by an average of 17.33% and 12.14% in two years compared to the CK, respectively. Therefore, the study and application of wood vinegar due to its compound effects on crop growth and yield is of great importance to sustainable agriculture, crop ecology, and environmental protection. [ABSTRACT FROM AUTHOR]

Simple Summary: During the development of animals from juvenile to adult, their behaviour, morphology, and physiology can be altered to accommodate specific developmental periods. Early development in young animals directly affects the acquisition and maturation of behavioural skills and neurological functions in adults. Studying the behavioural development of young animals can help reveal the formation and evolution of animal behaviour. As the only mammalian group that flies, studying the behavioural development of young bats can help improve our understanding of bat flight behaviour. From our observations in the laboratory, we found that wing flapping and wing spreading behaviours in young bats promote earlier flight attempts. Behavioural development is an important aspect of research on animal behaviour. In bats, many studies have been conducted on the development of flight behaviour, but the postnatal behavioural development of bats remains largely unexplored. We studied the behaviours and postnatal development of infant bats by conducting controlled video recorded experiments. Our results showed that before weaning, Asian parti-coloured bats (Vespertilio sinensis) were able to exhibit four types of behaviours, namely, crawling, head moving, wing flapping, and wing spreading, and these behaviours are different from those observed in experiments with adult bats. The number of occurrences of these behaviours was correlated with age and scaled mass index. Furthermore, the number of occurrences of these behaviours in young bats could also reflect their physical developmental status. In young bats, wing flapping and spreading might be a type of play behaviour. These behaviours were negatively correlated with the time of the first flight, indicating that they might help to promote individual physical development. Our results provide fundamental data for revealing the ontogenetic and neurophysiological mechanisms of behavioural development in bats. [ABSTRACT FROM AUTHOR]