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The article focuses on the impact of the size of pore packing materials for peptide separations in ultra performance liquid chromatography peptide mapping. The pore size materials of 130 A and 300 A are compared in the separation of its two complex peptide digests. The result shows that both peptide separation technology columns give useful separations of peptides over a wide range of sizes and chemical properties.

Crude synthetic peptide samples were separated on preparative Atlantis™ dc18 columns. Fraction collection was triggered by the mass-to-charge (rn/z) ratio of the peptides. which specified the target peptide in the collected fraction to increase the fraction purities. Mass-directed peptide purification was more robust than UV-based collection under overloading conditions, and increased the traction purities. LC-MS-MS analysis was further employed to identify the impurities in the crude synthetic peptide sample. This study demonstrates high mass loading, selective purification. high recoveries and purity, and the capability of utilizing mass-directed fractionation for purifying peptides on preparative RPLC columns. [ABSTRACT FROM AUTHOR]