1. 3D Printing as an Effective Quality Assurance Implementation in Massive-Scale SARS-CoV-2 Testing at a SwabSeq Next-Generation Sequencing Laboratory.
- Author
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Sathe, Laila M, Khan, Nishrat N, Williams, Jazmine M, Saul, Rosita, Jajieh, Kane, Sartippour, Maryam R, Young, Rachel, Xie, Joanna, Marquette, Dawn M, Duncan, Tiffany, Eskin, Eleazar, and Arboleda, Valerie A
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PATHOLOGICAL laboratories , *COVID-19 , *SEQUENCE analysis , *QUALITY assurance , *HUMAN error , *THREE-dimensional printing , *COVID-19 testing , *POLYMERASE chain reaction - Abstract
Massive-scale SARS-CoV-2 testing using the SwabSeq diagnostic platform came with quality assurance challenges due to the novelty and scale of sequencing-based testing. The SwabSeq platform relies on accurate mapping between specimen identifiers and molecular barcodes to match a result back to a patient specimen. To identify and mitigate mapping errors, we instituted quality control using placement of negative controls within a rack of patient samples. We designed 2-dimensional paper templates to fit over a 96-position rack of specimens with holes to show the control tube placements. We designed and 3-dimensionally printed plastic templates that fit onto 4 racks of patient specimens and provide accurate indications of the correct control tube placements. The final plastic templates dramatically reduced plate mapping errors from 22.55% in January 2021 to less than 1% after implementation and training in January 2021. We show how 3D printing can be a cost-effective quality assurance tool to mitigate human error in the clinical laboratory. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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