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The objective of this paper was to report a bacterium designated as 88D, capable of producing poly (3-hydroxybutyrate-co-3-hydroxyvalerate) [P (3HB-co-3HV)] copolymer from a single carbon source, which was isolated from a municipal sewage treatment plant in Hyderabad, India. This microorganism, based on the phenotypical features and genotypic investigations, was identified as Bacillus sp. The optimal growth of Bacillus sp. 88D occurred between 28 and 30°C and at pH 7. The strain yielded a maximum of 64.62% dry cell weight (DCW) polymer in the medium containing glucose as carbon source, which was followed by 60.46% DCW polymer in glycerol containing medium. Bacillus sp. 88D produced P (3HB-co-3HV) from glucose or glycerol, when they were used as a single carbon substrate. This bacterium produced polyhydrxybutyrate (PHB) when sodium acetate was used as sole carbon substrate. The viscosity average molecular mass (Mv) of the copolymers ranged from 523 to 627 kDa. The physical, chemical and mechanical properties of the biopolymers were characterized. [ABSTRACT FROM AUTHOR]