1. Designer Functionalized Self-Assembling Peptide Scaffolds for Adhesion, Proliferation, and Differentiation of MC3T3-E1.
- Author
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Lu, Tingli, Chen, Ting, Zhai, Yuankun, Ma, Yufan, and Xiao, Yuehong
- Subjects
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MOLECULAR self-assembly , *PEPTIDES , *HYDROPHOBIC compounds , *NANOSTRUCTURED materials , *BONE growth , *CELL proliferation - Abstract
Induction of some specific cell signaling molecules in peptide scaffold play a critical role in developing of functional substitutes for damaged tissue. In this study, a new peptide ELK8 (Ac-LELELKLK-CONH2) was designed by replacing Ala in EAK16 (Ac-AEAEAKAKAEAEAKAK-CONH2) with more hydrophobic residue Leu in order to enhance the strength of peptide. The functionalized peptides were obtained through directly coupling three functional motifs to C-terminal of ELK8, such as osteogenic growth peptide, osteopontin cell adhesion motif, and 2-unit RGD binding sequence. Then, the new self-assembling peptide scaffolds were fabricated by mixing three functionalized peptides severally with pure ELK8 at the ratio of 1:1 (v:v) and the nanostructures of self-assembling peptides were investigated with AFM and DLS. The osteogenic efficacy of designed peptides on mouse pre-osteoblast MC3T3-E1 were examined after cells were incubated in scaffolds for 14 d. The results showed that these functionalized peptides, ALK mix particularly, promoted the attachment, proliferation, and osteogenic differentiation of MC3T3-E1 cells by increasing in cell numbers, ALP activities and osteocalcin concentrations. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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