1. MiR-186 inhibits cell proliferation and invasion in human cutaneous malignant melanoma.
- Author
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Su, Bei-bei, Zhou, Shu-wei, Gan, Cai-bin, and Zhang, Xiao-ning
- Subjects
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MELANOMA , *CANCER cell proliferation , *MICRORNA , *REVERSE transcriptase polymerase chain reaction , *APOPTOSIS , *METASTASIS , *RNA , *CELL physiology , *GENE expression , *CELL cycle , *CELL motility , *CUTANEOUS malignant melanoma , *GENES , *GENETIC techniques , *CELL lines - Abstract
Aims: MicroRNA-186 (miR-186) has been shown to be involved in various types of cancer. The purpose of this study was to investigate the expression level and functional role of miR-186 in human cutaneous malignant melanoma cells.Subjects and Methods: Expression of miR-186 was analyzed in human cutaneous malignant melanoma (CMM) cell lines SK-MEL-1, G-361, A375 and A875, and human normal epidermal melanocytes cell line HEMn-LP by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Additionally, the functional role of miR-186 on melanoma cells was investigated by transfection of miR-186 mimic followed by analyses of cell proliferation, apoptosis, and metastasis.Results: We found that the expression levels of miR-186 were decreased in CMM cell lines compared with normal epidermal melanocytes cell line. Moreover, overexpression of miR-186 inhibited cells proliferation through abrogating the G1-S transition, and reduced cells migration and invasion.Conclusions: Our findings suggested that miR-186 exhibit an inhibitory effect on CMM cell proliferation, migration, and invasion; thus, may serve as a potential therapeutic target for human CMM intervention. [ABSTRACT FROM AUTHOR]- Published
- 2018
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