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In the mid-1970s, the first-generation sequencing technique (Sanger) was created. It used Advanced BioSystems sequencing devices and Beckman's GeXP genetic testing technology. The second-generation sequencing (2GS) technique arrived just several years after the first human genome was published in 2003. 2GS devices are very quicker than Sanger sequencing equipment, with considerably cheaper manufacturing costs and far higher throughput in the form of short reads. The third-generation sequencing (3GS) method, initially introduced in 2005, offers further reduced manufacturing costs and higher throughput. Even though sequencing technique has result generations, it is error-prone due to a large number of reads. The study of this massive amount of data will aid in the decoding of life secrets, the detection of infections, the development of improved crops, and the improvement of life quality, among other things. This is a challenging task, which is complicated not just by a large number of reads and by the occurrence of sequencing mistakes. As a result, error correction is a crucial duty in data processing; it entails identifying and correcting read errors. Various k-spectrum-based error correction algorithms' performance can be influenced by a variety of characteristics like coverage depth, read length, and genome size, as demonstrated in this work. As a result, time and effort must be put into selecting acceptable approaches for error correction of certain NGS data. [ABSTRACT FROM AUTHOR]

Cardiac arrhythmia is an illness in which a heartbeat is erratic, either too slow or too rapid. It happens as a result of faulty electrical impulses that coordinate the heartbeats. Sudden cardiac death can occur as a result of certain serious arrhythmia disorders. As a result, the primary goal of electrocardiogram (ECG) investigation is to reliably perceive arrhythmias as life-threatening to provide a suitable therapy and save lives. ECG signals are waveforms that denote the electrical movement of the human heart (P, QRS, and T). The duration, structure, and distances between various peaks of each waveform are utilized to identify heart problems. The signals' autoregressive (AR) analysis is then used to obtain a specific selection of signal features, the parameters of the AR signal model. Groups of retrieved AR characteristics for three various ECG kinds are cleanly separated in the training dataset, providing high connection classification and heart problem diagnosis to each ECG signal within the training dataset. A new technique based on two-event-related moving averages (TERMAs) and fractional Fourier transform (FFT) algorithms is suggested to better evaluate ECG signals. This study could help researchers examine the current state-of-the-art approaches employed in the detection of arrhythmia situations. The characteristic of our suggested machine learning approach is cross-database training and testing with improved characteristics. [ABSTRACT FROM AUTHOR]