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Your search keyword '"Guo, Fan"' showing total 2 results
2 results on '"Guo, Fan"'

1. Pterostilbene, a Bioactive Component of Blueberries, Alleviates Renal Interstitial Fibrosis by Inhibiting Macrophage-Myofibroblast Transition.

Author

Feng, Yanhuan, Guo, Fan, Mai, Hongxia, Liu, Jing, Xia, Zijing, Zhu, Guonian, Zhang, Jinhang, Ma, Liang, and Fu, Ping

Subjects
*BLUEBERRIES, *ANIMAL experimentation, *BIOLOGICAL models, *BONE marrow, *CELL physiology, *STATISTICAL correlation, *CYTOKINES, *FIBROBLASTS, *FLOW cytometry, *GENE expression, *IMMUNOHISTOCHEMISTRY, *KIDNEY diseases, *MACROPHAGES, *MICE, *MUSCLE proteins, *ORGANIC compounds, *POLYMERASE chain reaction, *STATISTICAL hypothesis testing, *STATISTICS, *T-test (Statistics), *TRANSCRIPTION factors, *TRANSFORMING growth factors-beta, *URETERIC obstruction, *WESTERN immunoblotting, *PHYTOCHEMICALS, *DATA analysis, *FIBROSIS, *RESVERATROL, *REVERSE transcriptase polymerase chain reaction, *DATA analysis software, *GENE expression profiling, *FLUOROIMMUNOASSAY, *IN vitro studies, *ONE-way analysis of variance, *IN vivo studies
Abstract

Pterostilbene (PTB) is a derivative of resveratrol present in grapes and blueberries. PTB is structurally similar to resveratrol, possessing properties such as being analgesic, anti-aging, antidiabetic, anti-inflammatory, anti-obesity, anti-oxidation, cholesterol-reductive, and neuroprotective. However, there have not been reports on the effect of PTB on macrophage-myofibroblast transition (MMT) induced fibrosis in kidney. In this study, we investigated the antifibrotic effects of PTB on the in vivo mouse unilateral ureteral obstruction (UUO) model and in vitro MMT cells. Kidneys subjected to UUO with PTB treatment were collected for the investigation of PTB mediating MMT derived renal interstitial fibrosis. We conducted kidney RNA-seq transcriptomes and TGF- β 1-induced bone marrow-derived macrophages assays to determine the mechanisms of PTB. We found that PTB treatment suppressed the interstitial fibrosis in UUO mice. PTB also attenuated the number of MMT cells in vivo and in vitro. The transcriptomic analysis showed that CXCL10 may play a central role in the process of PTB-treated renal fibrosis. The siRNA-mediated CXCL10 knockdown decreased the number of MMT cells in TGF- β 1-induced bone marrow-derived macrophages. Our results suggested that PTB attenuated renal interstitial fibrosis by mediating MMT by regulating transcriptional activity of CXCL10. [ABSTRACT FROM AUTHOR]

Published
2020
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2. 2-Arachidonylglycerol Protects Primary Astrocytes Exposed to Oxygen-Glucose Deprivation Through a Blockade of NDRG2 Signaling and STAT3 Phosphorylation.

Author

Wang, Feng, Li, Mo, Li, Xin, Kinden, Renee, Zhou, Heng, Guo, Fan, Wang, Qiang, and Xiong, Lize

Subjects
*GLYCERIN, *ASTROCYTES, *ARACHIDONIC acid, *CEREBRAL ischemia, *PHOSPHORYLATION, *GENE expression, *CELL metabolism, *ANIMAL experimentation, *ANIMAL populations, *BIOCHEMISTRY, *CARRIER proteins, *CELL culture, *CELL physiology, *CELLS, *CELLULAR signal transduction, *DRUGS, *DOSE-effect relationship in pharmacology, *GENES, *GENETIC techniques, *GLUCOSE, *GLYCERIDES, *PHENOMENOLOGY, *NERVE tissue proteins, *NEUROTRANSMITTERS, *PHARMACOLOGY, *PREVENTIVE health services, *RATS, *PHARMACODYNAMICS, *PREVENTION
Abstract

The human N-Myc downstream-regulated gene 2 (NDRG2) is expressed in astrocytes, and may be involved in the modulation of gliacyte function in the central nervous system. Our previous study found suppression of NDRG2 up-regulation in reactive astrocytes in cerebral ischemic tolerance. 2-Arachidonylglycerol (2-AG) can induce cerebral ischemic tolerance. However, the underlying mechanism of NDRG2 in cytoprotection induced by 2-AG in primary astrocytesis still unknown. In this study, we investigated the role of NDRG2 in cerebral ischemic tolerance induced by 2-AG after oxygen-glucose deprivation (OGD) in primary astrocytes. The results showed that primary astrocytes exposed to OGD resulted in marked increase of lactate dehydrogenase (LDH) release and decrease of methyl thiazolyl tetrazolium (MTT) reduction activity in comparison to control cultures. The levels of NDRG2 and phospho-signal transducer and activator of transcription 3 (pSTAT3) in the OGD group were comparably higher than those in the control group, and the up-regulation of NDRG2 and pSTAT3 was suppressed in NDRG2 siRNA group. The cell viability in the 2-AG group was higher than that in the OGD group, and transfecting the NDRG2 pSRL-CDH1-GFP vector reversed the protective effects of 2-AG. The levels of NDRG2 and pSTAT3 in the 2-AG group were lower than those in the OGD group. 2-AG suppressed STAT3 phosphorylation by decreased expression of NDRG2. In conclusion, 2-AG protects primary astrocytes exposed to oxygen-glucose deprivation through a blockade of NDRG2 signaling and STAT3 phosphorylation. These findings bring insight to the roles of NDRG2 in ischemic-hypoxic injury and provide novel potential targets for future potent clinical therapies on cerebral ischemia injury. [ABSTRACT FROM AUTHOR]

Published
2016
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