1. Novel enzyme-linked immunoassay based on nanoceria as colorimetric substrate for foodborne pathogen detection.
- Author
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Zhan, Lei, Zhou, Jia Ying, Li, Chun Mei, Li, Yuan Fang, and Huang, Cheng Zhi
- Subjects
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FOOD pathogens , *IMMUNOASSAY , *GLUCOSE oxidase , *ENZYME-linked immunosorbent assay , *HORSERADISH peroxidase , *PEROXIDASE - Abstract
A reliable and robust colorimetric assay for highly selective detection of intact S. aureus employing nanoceria as chromatic agents. [Display omitted] • Glucose oxidase was employed to in situ produce H 2 O 2 in immunoassay. • Stable nanoceria was used as quantitative chromogenic indicators. • Capture antibody-bacteria-aptamer configuration was adopted in this assay. • The immunoassay has demonstrated functionality for S. aureus detection in real samples. Rapid and reliable detection of foodborne pathogens is a key requirement for food safety. Traditional rapid ELISA methods assessed by the ability of horseradish peroxidase to oxidize a chromogenic substrate such as 3, 3′, 5, 5′-tetramethylbenzidine in the presence of H 2 O 2 may result in the high rate of negative responses because of the instability of H 2 O 2 and product. Here, we report the use of redox active nanoceria as colorimetric indicator in immunoassay taking Staphylococcus aureus as a model. The nanoceria is fully water-soluble and can be stored as least 90 days in the refrigerator while maintaining the same performance. Moreover, it does not require external H 2 O 2 , as glucose oxidase used in the immunoassay can catalyze the oxidation of glucose to produce H 2 O 2 in situ , which is more robust in signal readout. Taken together, these results demonstrate that nanoceria are potentially suitable as colorimetric substrate in the development of novel enzyme immunoassays. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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