1. A GFP-expressing minigenome of a chrysovirus replicating in fungi.
- Author
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Domènech-Eres, Robert, Jaeckel, Mareike, Hadeler, Birgit, Lienemann, Tim, Lutz, Tobias, and Heinze, Cornelia
- Subjects
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FUNGAL viruses , *VIROLOGY , *CELL imaging , *DOUBLE-stranded RNA , *FUNGI , *CIS-regulatory elements (Genetics) , *FUSARIUM - Abstract
The Fusarium graminearum virus China 9 (FgV-ch9) is a member of the genus Betachrysovirus in the Chrysoviridae family and causes hypovirulence in its host, Fusarium graminearum , the causal agent of Fusarium head blight. Although insights into viral biology of FgV-ch9 have expanded in recent years, questions regarding the function of virus-encoded proteins, cis-acting elements, and virus transmission are yet to be answered. Therefore, we developed a tool for the establishment of an artificial 6th segment of FgV-ch9, which encodes a GFP gene flanked by the non-translated regions of FgV-ch9 segment 1. Subsequently, we have proved successful encapsidation of this artificial segment into virus particles as well as its horizontal transmission. Expression of GFP was further verified via immunoassay and life cell imaging. Thus far, we were able to establish for the first time a mini-replicon system for segmented dsRNA viruses replicating in fungi. • An artificial virus transcript is converted to dsRNA by the chrysovirus FgV-ch9. • The artificial dsRNA segment is encapsidated into virus particles. • The artificial dsRNA is horizontally transmitted. • The artificial dsRNA is suitable for subcellular localization studies. • The artificial dsRNA is suitable for the investigation of cis -acting elements. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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