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Pentachlorophenol (PCP) is a persistent organic pollutant usually present in the form of sodium salts (PCP-Na) that has been banned for many years, but it can still be detected in animal food. The present study established a method of detecting PCP-Na and its metabolites—tetrachlorocatechol (TCC), pentachlorophenol acetate (PCP-acetate), and pentachloroanisole (PCA)—in swine samples (pork, fat, liver, heart, lungs and kidney), simultaneously using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and gas chromatography-tandem mass spectrometry (GC-MS/MS) based on the modified QuEChERS pre-treatment method. The validation results exhibited a good sensitivity with limits of quantitation (LOQs) of 1 μg/kg–2 μg/kg. The recoveries of spiked samples were in the range of 60.5–119.9%, with a relative standard deviation (RSD) between replicates (n = 5) of between 0.70% and 12.06%. [ABSTRACT FROM AUTHOR]

• Fifty pesticides and eight metabolites in eggs were simultaneously detected. • Multi-functional filter based on QuEChERS were used to rapid sample preparation. • The efficiency was obviously improved with shorter cleanup time of < 1 min. • Acetamiprid, pyrimethanil and fipronil sulfone were detected in actual egg samples. A sensitive method for the simultaneous determination of 58 pesticides and relevant metabolites in eggs was developed using ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) after clean-up with a multi-functional filter (MFF) based on quick, easy, cheap, effective, rugged, and safe method (QuEChERS). The egg sample was extracted with 5 ml water and 10 ml 1% acetic acid in acetonitrile and then salt out with sodium chloride. The extracted solution was filtered directly through an MFF containing 50 mg PSA, 50 mg C18, and 150 mg magnesium sulphate before UHPLC-MS/MS analysis. The clean-up and filter procedures were integrated using the MFF to substantially improve the work efficiency. Good linearity was shown for each analyte, and all the correlation coefficients exceeded 0.99. The recoveries in the eggs at the five spiked levels were 74.4%–115.2%, and the relative standard deviations (RSDs) were less than 15.3%. The limit of detection (LOD) and the limit of quantitation (LOQ) of 58 pesticides and 8 metabolites in eggs were 0.1–1.0 μg/kg and 0.2–5.0 μg/kg, respectively. The decision limit (CCα) and detection capacity (CCβ) were 3.4–111.1 μg/kg and 6.8–122.1 μg/kg, respectively. This method has also been successfully applied in the determination of actual eggs samples. This developed method is more effective and faster in the monitoring of pesticide residue in eggs compared to the traditional analytical method. [ABSTRACT FROM AUTHOR]