1. Dynamic gene expression of southern rice black-streaked dwarf virus during its infection in rice plants and vector insects.
- Author
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Li, Shen, Huang, Jiayu, Wang, Ying, Yan, Jingya, Zhao, Yaling, Yang, Xin, Zhou, Guohui, and Zhang, Tong
- Subjects
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GENE expression , *INSECT-plant relationships , *INSECT genes , *VIRAL genes , *PLANTHOPPERS - Abstract
In this century, a disease caused by southern rice black-streaked dwarf virus (SRBSDV) has resulted in significant loss in rice production in Asia. Aside from infecting rice plants, SRBSDV is transmitted by white-backed planthopper (WBPH) in a persistent propagative manner. Recent studies showed that SRBSDV can dynamically modulate the host cells throughout the infection progress. However, the expression dynamics of the SRBSDV genes during infection remain unclear. Here we established an absolute real-time quantitative PCR method to assess the dynamic of the SRBSDV genes expression in rice plants and planthoppers. Apart from displaying the expression levels of viral genes, we discovered that the expression level of viral genes in insects significantly surpasses that in plant cells. In addition, we identified two nonstructural proteins with unknown functions that exhibit the highest expression levels in plant and insect cells, respectively, which provide possible targets for restraining the disease outbreaks. • The gene expression level of SRBSDV is significantly higher in insect than in plant. • SRBSDV P5-2 is most abundantly expressed in rice plants at the early infection stage. • P9-2 is the most highly expressed SRBSDV gene in the vector insects. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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