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We prove some instances of Wilkie's conjecture on the density of rational points on sets definable in the real exponential field. In particular, we prove that this conjecture is true for surfaces defined using restricted exponentiation, and that it is true for a general Pfaffian surface provided that the surface admits a certain kind of parameterization. [ABSTRACT FROM AUTHOR]

For many diseases the infection status of individuals cannot be observed directly, but can only be inferred from biomarkers that are subject to measurement error. Diagnosis of infection based on observed symptoms can itself be regarded as an imperfect test of infection status. The temporal relationship between infection and marker outcomes may be complex, especially for recurrent diseases where individuals can experience multiple bouts of infection. We propose an approach that first models the unobserved longitudinal infection status of individuals conditional on relevant covariates, and then jointly models the longitudinal sequence of biomarker outcomes conditional on infection status and covariate information through time, thus resulting in a joint model for longitudinal infection and biomarker sequences. This model can be used to investigate the temporal dynamics of infection, and to evaluate the usefulness of biomarkers for monitoring purposes. Our work is motivated and illustrated by a longitudinal study of bovine digital dermatitis (BDD) on commercial dairy farms in North West England and North Wales, in which the infection of interest is Treponeme spp., and the biomarkers of interest are a continuous enzyme-linked immunosorbent assay test outcome and a dichotomous outcome, foot lesion status. BDD is known to be one of the possible causes of foot lesions in cows. [ABSTRACT FROM AUTHOR]

Do American troops help or hinder economic growth in other countries? We consider a newly constructed dataset of the deployment of U.S. troops over the years 1950–2000 and discover a positive relationship between deployed troops and host country economic growth, which is robust to multiple control variables. Each tenfold increase in U.S. troops is associated with a one–third percentage point increase in average host country annual growth. We explore three possible causal explanations: a Keynesian aggregate demand boost; the diffusion of institutions; and security. Extensive econometric testing, including the use of panel data, confirms the core relationship. [ABSTRACT FROM PUBLISHER]

We report a case of nephropleural fistula causing empyema and respiratory failure in a 68-year-old gentleman with a long history of urological problems including recurrent nephrolithiasis and urinary tract infections. He was admitted with sepsis, a productive cough, pyuria, and reduced breath sounds over the left hemithorax. Radiological imaging revealed a fistulous connection between a left-sided perinephric abscess and the pleural space. He was commenced on broad spectrum intravenous antibiotics but developed progressive respiratory failure requiring intensive care admission. Urinary and pleural aspirates cultured facultative anaerobic pathogens with identical resistance patterns. Drainage of thoracic and perinephric collections was carried out, allowing him to be extubated after 24 hours and discharged home after 18 days on an extended course of oral antibiotics. Left nephrectomy is now planned after a period of convalescence. Empyema developing in patients with known urolithiasis should alert the treating physician to the possibility that a pathological communication has formed especially if typical urinary tract pathogens are cultured from respiratory sampling. [ABSTRACT FROM AUTHOR]

The dipeptidyl peptidase-IV inhibitor saxagliptin (Onglyza) can undergo a thermodynamically favored cyclization to form the corresponding cyclic amidine. The kinetics and mechanism of this conversion were examined to develop a commercial synthesis that afforded saxagliptin with only trace levels of this key byproduct. Important findings of this work are the identification of a profound solvent effect and the determination of an autocatalytic pathway. Both of these phenomena result from transition structures involving proton transfer. [ABSTRACT FROM AUTHOR]

Phasic dopamine (DA) transmission encodes the value of reward-predictive stimuli and influences both learning and decision-making. Altered DA signaling is associated with psychiatric conditions characterized by risky choices such as pathological gambling. These observations highlight the importance of understanding how DA neuron activity is modulated. While excitatory drive onto DA neurons is critical for generating phasic DA responses, emerging evidence suggests that inhibitory signaling also modulates these responses. To address the functional importance of inhibitory signaling in DA neurons, we generated mice lacking the β3 subunit of the GABAA receptor specifically in DA neurons (β3-KO mice) and examined their behavior in tasks that assessed appetitive learning, aversive learning, and risk preference. DA neurons in midbrain slices fromβ3-KO mice exhibited attenuated GABA-evoked IPSCs. Furthermore, electrical stimulation of excitatory afferents to DA neurons elicited more DA release in the nucleus accumbens of β3-KO mice as measured by fast-scan cyclic voltammetry. β3-KO mice were more active than controls when given morphine, which correlated with potential compensatory upregulation of GABAergic tone onto DA neurons. β3-KO mice learned faster in two food-reinforced learning paradigms, but extinguished their learned behavior normally. Enhanced learning was specific for appetitive tasks, as aversive learning was unaffected in β3-KO mice. Finally, we found that β3-KO mice had enhanced risk preference in a probabilistic selection task that required mice to choose between a small certain reward and a larger uncertain reward. Collectively, these findings identify a selective role for GABAA signaling in DA neurons in appetitive learning and decision-making. [ABSTRACT FROM AUTHOR]

We demonstrate a signal-processing concept for imaging the sky at millisecond rates with radio interferometers. The "Pocket Correlator" (PoCo) correlates the signals from multiple elements of a radio interferometer fast enough to image brief, dispersed pulses. By the nature of interferometry, a millisecond correlator functions like a large, single-dish telescope, but with improved survey speed, spatial localization, calibration, and interference rejection. To test the concept, we installed PoCo at the Allen Telescope Array (ATA) to search for dispersed pulses from the Crab pulsar, B0329+54, and M31 using total-power, visibility-based, and image-plane techniques. In 1.7 hr of observing, PoCo detected 191 giant pulses from the Crab pulsar brighter than a typical 5σ sensitivity limit of 60 Jy over pulse widths of 3 ms. Roughly 40% of pulses from pulsar B0329+54 were detected by using novel visibility-based techniques. Observations of M31 constrain the rate of pulses brighter than 190 Jy in a three-degree region surrounding the galaxy to <4.3 hr-1. We calculate the computational demand of various visibility-based pulse search algorithms and demonstrate how compute clusters can help meet this demand. Larger implementations of the fast imaging concept will conduct blind searches for millisecond pulses in our Galaxy and beyond, providing a valuable probe of the interstellar/intergalactic media, discovering new kinds of radio transients, and localizing them to constrain models of their origin. [ABSTRACT FROM AUTHOR]

Arid and semi-arid environments are characterized by extreme fluctuations in temperature and low rainfall, which present significant challenges to the animals inhabiting these areas. However, the presence of burrows may allow animals to avoid climatic extremes and predators and may act as valuable foraging sites. We assessed the microhabitat conditions (maximum and minimum temperature, relative humidity and seed abundance) of aardvark (Orycteropus afer) burrows in relation to paired non-burrow areas at three sites in South Africa. We also describe the extent to which they are used as resources by other vertebrates. Maximum temperatures were significantly lower and minimum temperatures and relative humidity values were significantly higher inside the burrows than outside. The concentration of seeds inside the burrows and at the paired non-burrow sites was similar. Twenty-seven vertebrate species (21 mammals, two birds, three reptiles and one amphibian) were recorded making use of the burrows and it is likely that these species accrue benefits (e.g. a buffered microclimate) from burrow use. However, our sampling was biased towards mammals and nocturnal species. Consequently, we recommend further work to establish the overall reliance of vertebrate taxa on aardvark burrows in arid and semi-arid environments. [ABSTRACT FROM AUTHOR]

Purpose: To develop and psychometrically evaluate two questionnaires measuring both positive and negative postnatal health of mothers (M-PHI) and fathers (F-PHI) during the first year of parenting. Methods: The M-PHI and the F-PHI were developed in four stages. Stage 1: Postnatal women's focus group (M-PHI) and postnatal fathers' postal questionnaire (F-PHI); Stage 2: Qualitative interviews; Stage 3: Pilot postal survey and main postal survey; and Stage 4: Test-retest postal survey. Results: The M-PHI consisted of a 29-item core questionnaire with six main scales and five conditional scales. The F-PHI consisted of a 27-item questionnaire with six main scales. All scales achieved good internal reliability (Cronbach's α 0.66-0.87 for M-PHI, 0.72-0.90 for F-PHI). Intraclass correlation coefficients demonstrated high test-retest reliability (0.60-0.88). Correlation coefficients supported the criterion validity of the M-PHI and the F-PHI when tested against the Short-Form-12 (SF-12), Edinburgh Postnatal Depression Scale (EPDS) and the Warwick and Edinburgh Mental Well-Being Scale (WEMWBS). Conclusion: The M-PHI and F-PHI are valid, reliable, parent-generated instruments. These unique instruments will be invaluable for practitioners wishing to promote family-centred care and for trialists and other researchers requiring a validated instrument to measure both positive and negative health during the first postnatal year, as to date no such measurement has existed. [ABSTRACT FROM AUTHOR]

Tropical coral reef teleosts are exclusively ectotherms and their capacity for physical and physiological performance is therefore directly influenced by ambient temperature. This study examined the effect of increased water temperature to 3 °C above ambient on the swimming and metabolic performance of 10 species of damselfishes (Pomacentridae) representing evolutionary lineages from two subfamilies and four genera. Five distinct performance measures were tested: (a) maximum swimming speed ( Ucrit), (b) gait-transition speed (the speed at which they change from strictly pectoral to pectoral-and-caudal swimming, Up−c), (c) maximum aerobic metabolic rate (MO2−MAX), (d) resting metabolic rate (MO2−REST), and (e) aerobic scope (ratio of MO2−MAX to MO2−REST, ASC). Relative to the control (29 °C), increased temperature (32 °C) had a significant negative effect across all performance measures examined, with the magnitude of the effect varying greatly among closely related species and genera. Specifically, five species spanning three genera ( Dascyllus, Neopomacentrus and Pomacentrus) showed severe reductions in swimming performance with Ucrit reduced in these species by 21.3-27.9% and Up−c by 32.6-51.3%. Furthermore, five species spanning all four genera showed significant reductions in metabolic performance with aerobic scope reduced by 24.3-64.9%. Comparisons of remaining performance capacities with field conditions indicate that 32 °C water temperatures will leave multiple species with less swimming capacity than required to overcome the water flows commonly found in their respective coral reef habitats. Consequently, unless adaptation is possible, significant loss of species may occur if ocean warming of ≥3 °C arises. [ABSTRACT FROM AUTHOR]

NMDA receptors (NMDARs) contribute to phasic transmission and synaptic plasticity and are thought to be important for learning. To better understand where NMDAR signaling is necessary for learning, we combined viral genetic strategies with genetic mouse models to investigate the contribution of NMDARs in the dopamine system to appetitive Pavlovian conditioning. NMDAR signaling in dopamine neurons was not required for Pavlovian conditioning; however, NMDARs in D1 dopamine receptor (D1R)-expressing medium spiny neurons (MSNs), which receive input from dopamine neurons, were critical for this type of learning. NMDAR signaling was also required in brain regions that project to dopamine neurons, because removing NMDARs from afferent neurons to the ventral tegmental area (VTA) also prevented learning. This effect was likely attributable to loss of NMDAR signaling in the neurons of the prefrontal cortex (PFC), because learning could be restored in these animals by rescuing NMDAR expression in the PFC. Moreover, removing NMDARs exclusively from the PFC also prevented learning. Our findings suggest that NMDARs in neurons that project to and receive projections from the VTA are necessary for Pavlovian conditioning and specifically implicate the PFC and D1R-expressing MSNs in associative learning. [ABSTRACT FROM AUTHOR]

Objective:To describe our experience of a new multidisciplinary balance clinic for the management of patients with vestibular disorders, run as a pilot project for 12 months.Design:Retrospective review of the outcomes of 194 patients.Subjects:All patients were first evaluated at the balance assessment clinic. Subsequently, each case was discussed at the multidisciplinary balance clinic weekly review meeting, and management decisions made.Results:The mean waiting time for the balance assessment clinic was 12 weeks (standard deviation six weeks). In total, 74 per cent of patients underwent rehabilitation, 26 per cent were seen in the balance specialist clinic, 15 per cent underwent additional testing, 6 per cent were followed up in adult otology clinics and one patient was listed for surgery. The waiting time for vestibular rehabilitation was reduced from 21 to 15 weeks. Patient satisfaction with the service was encouraging, and no adverse outcomes were recorded.Conclusion:This multidisciplinary balance clinic, run by allied health professionals, represents an alternative model for the management of patients with balance disorders. [ABSTRACT FROM PUBLISHER]

UK national guidelines recommend the measurement of TSH receptor antibodies (TRAb) in certain clinical scenarios. A commercial third-generation TRAb autoantibody M22-biotin ELISA assay was introduced in May 2008 in our centre. To evaluate the diagnostic performance of a TRAb assay in a retrospective and subsequently a prospective cohort in a UK centre. A retrospective review of patients with thyroid disease followed by a prospective observational study in consecutive patients with newly found suppressed serum thyrotrophin (TSH). Medical records of 200 consecutive patients with thyroid disorders who had TRAb measured since the introduction of the assay. In a prospective study 44 patients with newly identified hyperthyroidism (TSH < 0·02 mIU/l) had sera assayed for TRAb prior to their clinic appointment at which a final diagnosis was sought. In the retrospective cohort, the manufacturer's cut-off point of TRAb ≥0·4 U/l resulted in a positive predictive value (PPV) of 95%, sensitivity 85%, specificity 94% and negative predictive value (NVP) 79% to diagnose Graves' disease using defined criteria. Receiver operating characteristic (ROC) analysis determined an optimal cut-off point of TRAb ≥3·5 U/l with a 100% specificity to exclude patients without Graves' disease at the cost though of a lower sensitivity (43%). In the prospective study, the sensitivity, PPV, specificity and NPV were all 96% using the ≥0·4 U/l cut-off. When combining hyperthyroid patients from both cohorts the assay sensitivity and specificity at ≥0·4 U/l cut-off were 95% and 92% respectively. A positive TRAb result increased the probability of Graves' disease for a particular patient by 25-35% and only six (2·5%) patients had a diagnosis of hyperthyroidism of uncertain aetiology after TRAb testing. The assay studied specifically identifies patients with Graves' disease. It is a reliable tool in the initial clinical assessment to determine the aetiology of hyperthyroidism and has the potential for cost-savings. [ABSTRACT FROM AUTHOR]

Background Presenteeism often precedes and follows a period of work absence. Cross-sectional analysis of a workforce survey highlighted health, psychosocial and work characteristics as being particularly important in understanding current work performance, but it is unclear whether these variables predict future work performance.Aims To establish whether self-reported health, perceptions of work and objective characteristics of work measured at baseline can predict performance at 6 months follow-up.Methods Self-completed questionnaires to assess health, objective characteristics of work and perceptions of work were completed at two public sector organizations. Follow-up questionnaires were completed at 6 months to assess workplace performance using a visual analogue scale for self-rated performance and the Stanford Presenteeism Scale 6 (SPS6).Results Five hundred and five employees completed questionnaires at baseline and 310 (61%) of these completed follow-up questionnaires. Psychological distress as measured with the General Health Questionnaire and perceptions of work predicted both self-rated performance and SPS6 score. Objective characteristics of work were relatively unimportant in the prediction of future performance.Conclusions This study has provided an initial indication of the factors that may predict performance at follow-up in the population studied. These findings may be used to generate hypotheses for future studies and highlights the need to assess a range of factors in relation to an individual's performance at work including health and perceptions of work. [ABSTRACT FROM AUTHOR]

Abstract: Biochar application to soil has been proposed as a mechanism for improving soil quality and the long term sequestration of carbon. The implications of biochar on pesticide behavior, particularly in the longer term, however, remains poorly understood. Here we evaluated the influence of biochar type, time after incorporation into soil, dose rate and particle size on the sorption, biodegradation and leaching of the herbicide simazine. We show that typical agronomic application rates of biochar (10–100 t ha−1) led to alterations in soil water herbicide concentrations, availability, transport and spatial heterogeneity. Overall, biochar suppressed simazine biodegradation and reduced simazine leaching. These responses were induced by a rapid and strong sorption of simazine to the biochar which limits its availability to microbial communities. Spatial imaging of 14C-labeled simazine revealed concentrated hotpsots of herbicide co-localized with biochar in the soil profile. The rate of simazine mineralization, amount of sorption and leaching was inversely correlated with biochar particle size. Biochar aged in the field for 2 years had the same effect as fresh biochar on the sorption and mineralization of simazine, suggesting that the effects of biochar on herbicide behavior may be long lasting. We conclude that biochar application to soil will reduce the dissipation of foliar applied pesticides decreasing the risk of environmental contamination and human exposure via transfer in the food chain, but may affect the efficacy of soil-applied herbicides. [Copyright &y& Elsevier]

Introduction: The association between hormonal contraceptive use and bone mineral density remains controversial. Hypothesis: Hormonal contraceptive use is positively associated with bone mass in young premenopausal women. Methods: Cross-sectional analysis of data collected from women aged 26-36 years ( n = 687) in the Childhood Determinants of Adult Health study-a longitudinal study investigating childhood determinants of cardiovascular disease, diabetes, and other chronic diseases in adulthood. Participants were not currently pregnant or breast-feeding. Contraceptive use was obtained by self-administered questionnaire. Women were categorized as combined oral contraceptive users ( n = 219), progestogen-only contraceptive users ( n = 43), and non-users of hormonal contraceptives ( n = 425). Bone mass was measured by quantitative ultrasound. Results: Compared with women who were not using any hormonal contraceptives, women using combined oral contraceptives had significantly higher values of broadband ultrasound attenuation (BUA), speed of sound, and quantitative ultrasound index. These associations remained after adjustment for confounders. Progestogen-only contraceptive users had higher BUA than non-users, but the differences were not statistically significant in this small group. Conclusion: Combined oral contraceptive use was associated with higher bone mass measured by quantitative ultrasound in this population-based sample of premenopausal women aged 26-36 while progestogen-only contraceptives appeared to have no deleterious effect on bone mass. [ABSTRACT FROM AUTHOR]

Abstract: Dopamine-producing neurons fire with both basal level tonic patterns and phasic bursts. Varying affinities of the five dopamine receptors have led to a hypothesis that higher affinity receptors are primarily activated by basal level tonic dopamine, while lower affinity receptors may be tuned to be sensitive to higher levels caused by phasic bursts. Genetically modified mice provide a method to begin to probe this hypothesis. Here we discuss three mouse models. Dopamine-deficient mice were used to determine which behaviors require dopamine. These behaviors were then analyzed in mice lacking D1 receptors and in mice with reduced phasic dopamine release. Comparison of the latter two mouse models revealed a similar failure to learn about and respond normally to cues that indicate either a positive or negative outcome, giving support to the hypothesis that phasic dopamine release and the D1 receptor act in the same pathway. However, the D1 receptor likely has additional roles beyond those of phasic dopamine detection, because D1 receptor knockout mice have deficits in addition to what has been observed in mice with reduced phasic dopamine release. [ABSTRACT FROM AUTHOR]

We characterize the physical properties of star-formation-driven outflows in a sample of 29 local dwarf galaxies drawn from the Dwarf Galaxy Survey. We made use of Herschel/PACS archival data to search for atomic outflow signatures in the wings of individual [CII] 158 μm spectra and in their stacked line profile. We find a clear excess of emission in the high-velocity tails of 11 sources, which can be explained with an additional broad component (tracing the outflowing gas) in the modeling of their spectra. The remaining objects are likely hosts of weaker outflows that can still be detected in the average stacked spectrum. In both cases, we estimate the atomic mass outflow rates which result to be comparable with the star-formation rates of the galaxies, implying mass-loading factors (i.e., outflow efficiencies) of the order of unity. Outflow velocities in all the 11 galaxies with individual detections are larger than (or compatible with) the escape velocities of their dark matter halos, with an average fraction of 40% of gas escaping into the intergalactic medium (IGM). Depletion timescales due to outflows are lower than those due to gas consumption by star formation in most of our sources, ranging from one hundred million to a few billion years. From the energetic point of view, our outflows are mostly consistent with momentum-driven winds generated by the radiation pressure of young stellar populations on dust grains, although the energy-driven scenario is not excluded if considering a coupling efficiency up to 20% between the energy injected by supernovae and the interstellar medium. Overall, our results suggest that, despite their low efficiencies, galactic outflows can regulate the star-formation history of dwarf galaxies. Specifically, they are able to enrich with metals the circumgalactic medium of these sources, bringing on average a non-negligible amount of gas into the IGM, where it will no longer be available for new star formation. Our findings are suitable for tuning chemical evolution models attempting to describe the physical processes shaping the evolution of dwarf galaxies. [ABSTRACT FROM AUTHOR]

The article discusses the family history and legal disputations surrounding the English Barony of Roos, focusing particularly on the 16th and 17th centuries. In 1526, Thomas Manners, 13th Baron Roos, was created Earl of Rutland. However, the death of Edward, third Earl of Rutland, without male issue resulted in the earldom passing to his brother John. However, the barony was allowed to pass through the female line, and the estate was divided in an effort to support his daughter Elizabeth's dignity as heir to the barony. The legal petitions, claims, and counter-petitions regarding the inheritance and succession are detailed.

During Pavlovian conditioning, phasic dopamine (DA) responses emerge to reward-predictive stimuli as the subject learns to anticipate reward delivery. This observation has led to the hypothesis that phasic dopamine signaling is important for learning. To assess the ability of mice to develop anticipatory behavior and to characterize the contribution of dopamine, we used a food-reinforced Pavlovian conditioning paradigm. As mice learned the cuereward association, they increased their head entries to the food receptacle in a pattern that was consistent with conditioned anticipatory behavior. Di-receptor knockout (D1R-KO) mice had impaired acquisition, and systemic administration of a D1R antagonist blocked both the acquisition and expression of conditioned approach in wild-type mice. To assess the specific contribution of phasic dopamine transmission, we tested mice lacking NMDA-type glutamate receptors (NMDARs) exclusively in dopamine neurons (NR1-KO mice). Surprisingly, NR1-KO mice learned at the same rate as their littermate controls. To evaluate the contribution of NMDARs to phasic dopamine release in this paradigm, we performed fast-scan cyclic voltammetry in the nucleus accumbens of awake mice. Despite having significantly attenuated phasic dopamine release following reward delivery. KO mice developed cueevoked dopamine release at the same rate as controls. We conclude that NMDARs in dopamine neurons enhance but are not critical for phasic dopamine release to behaviorally relevant stimuli; furthermore, their contribution to phasic dopamine signaling is not necessary for the development of cue-evoked dopamine or anticipatory activity in a D1R-dependent Pavlovian conditioning paradigm. [ABSTRACT FROM AUTHOR]

Background: Diabetes diagnosis is delayed 4–7 years and 50% are undiagnosed. Forty percent of hospitalized patients with any blood glucose level (BGL) ≥10 mmol/L have diabetes 3 months post-discharge, yet less than 5% are detected in hospital. We review identification of, and responses to, hyperglycaemia in inpatients at a teaching hospital. Methods: The world's largest retrospective review of medical records for inpatients with venous BGL ≥11.1 mmol/L without known diabetes over 12 months (2005–2006). The primary outcome was recognition of hyperglycaemia; secondary outcomes were treatment and documentation of follow up. Logistic regression was performed with variables including BGL, admitting team, length of stay and endocrine team review. Results: Of 10 973 people screened, 162 were eligible. The median age was 58 years and BGL 13.3 mmol/L, with increased mortality and length of stay. Hyperglycaemia was noted as definitely in 26%, maybe in 24% and definitely not in 50%. Forty percent of patients were treated in hospital and 19% on discharge. Follow up was documented for 24%. A higher BGL and review by the endocrine team were strongly associated with clinical recognition on uni- and multivariate analyses. However, where an endocrine review was sought for non-hyperglycaemia reasons, similar rates of non-recognition occurred. Conclusion: Despite evidence for improved inpatient outcomes when treated, and high short-term progression to frank diabetes, inpatient hyperglycaemia remains frequently missed. In-hospital recognition is cheap, and vital for the implementation of activities to improve outcomes and prevent progression and complications. Changes to systems for checking pathology results, medical officer education and inpatient screening guidelines are indicated. [ABSTRACT FROM AUTHOR]

Carbohydrate energy sources are known to be important for many adult parasitic wasps. Floral visitation is commonly observed, and in many groups specialized mouthpart morphology is associated with deep nectar extraction. In parasitic wasps, there are few data examining the relationship between host plant use and the length of the mouthparts. In an effort to associate mouthpart length to nectar source, pollen was identified from museum specimens of selected species of Agathirsia (Hymenoptera: Braconidae). Despite drastic differences in glossa length, the pollen of several species of Asteraceae were commonly found on specimens of various species. We infer that increased feeding efficiency, as opposed to nectar access, is the adaptive value of elongate glossae in Agathirsia. Species with short mouthparts, however, exhibited a difference from both the medium and long mouthpart categories in pollen similarity. This suggests that members of Agathirsia with elongated mouthparts are more apt to visit certain flowers compared with short-tongued species. Males and females exhibited considerable overlap of their common nectar sources and displayed similar pollen richness. Pollen richness was higher in species in the medium length category compared with the short category, but these results are confounded by the fact that the medium-length specimens were on average larger bodied. [ABSTRACT FROM AUTHOR]

There is continuing debate concerning the relationship between cigarette smoking and premature ovarian failure. The aim of this retrospective data analysis was to investigate whether smoking has a measurable effect on early follicular serum concentrations of inhibin B hormone, FSH and anti-Müllerian hormone (AMH) in women of reproductive age. A database containing data on age, smoking status and serum concentrations of inhibin B, FSH and AMH was analysed. Pearson's correlation coefficient was calculated to determine the correlation between hormone concentrations and age. One-way analysis of variance was used to determine any significant difference in age between smoking categories and a univariate general Linear model was used to compare geometric means and geometric mean ratios of hormone concentrations in relation to smoking status. Serum concentrations of inhibin B were significantly lower in women who had ever smoked cigarettes: F(2,332) = 3.371, P = 0.036. There was no statistically significant difference in FSH or AMH concentrations although a trend towards Lower AMH concentrations in smokers was observed. This analysis provides evidence of an advancement of ovarian ageing in women who smoke cigarettes and is relevant to women of childbearing age who wish to avoid premature decline in fertility. [ABSTRACT FROM AUTHOR]

A subjective novel approach incorporating an evidential reasoning (ER) algorithm is developed to achieve the risk estimation of ship hull vibration (SHV). A hierarchical structure for SHV modeling (hazard identification model) is constructed using a combined qualitative and quantitative approach. The quantitative criteria are converted to the qualitative criteria by applying a rule-based quantitative data transformation technique to make use of ER. A mapping process is formulated to convert and quantify the qualitative criteria. Intelligent Decision System (IDS) software is used for synthesis in the hierarchical structure and to produce the risk-estimation results graphically. The results of this paper reveal that the ER is capable of producing the risk estimation of SHV. [ABSTRACT FROM AUTHOR]

Background. GPs typically sanction absence from work by issuing sickness certificates. There has been some debate recently about changing the way sickness certificates are issued and by whom. However, without understanding GPs’ certification practices, their requirements in terms of training and education and how they feel the certification process should or should not be changed, measures aimed at improving the system are unlikely to succeed. [ABSTRACT FROM PUBLISHER]

Dessins d'enfants can be seen as bipartite graphs embedded in compact orientable surfaces. According to Grothendieck and others, a dessin uniquely determines a complex structure on the surface, even an algebraic structure as a projective algebraic curve defined over a number field. Combinatorial properties of the dessin should therefore determine the equations and also structural properties of the curve, such as the field of moduli or the field of definition. However, apart from a few series of examples, very few general results concerning such correspondences are known. As a step in this direction, we present a graph theoretic characterisation of certain quasiplatonic curves defined over cyclotomic fields, based on Wilson's operations on maps: these leave invariant the graph but change the cyclic ordering of edges around the vertices, therefore they change the embeddings, and hence the dessins, and hence the conformal and algebraic structure of the underlying curves. Under suitable assumptions, satisfied by many series of regular dessins, these changes coincide with the effect of Galois conjugation. This coincidence allows one to draw conclusions about Galois orbits and fields of definition of dessins. The possibilities afforded by these techniques, and their limitations, are illustrated by a new look at some known examples and a study of dessins based on the regular embeddings of complete graphs. [ABSTRACT FROM PUBLISHER]

Abstract: This paper brings together the results of five present-day studies of arable weed ecology, and applies these to the identification of past crop husbandry regimes on the basis of archaeobotanical weed assemblages. The contrasting husbandry regimes covered by the present-day studies include irrigation and dry farming, fallowing and rotation, intensive and extensive cultivation, and autumn and spring sowing. The weed floras associated with these regimes were analysed in terms of the functional ecology of the species represented. These same functional attributes, that distinguished contrasting regimes, were then measured for the weed species found accompanying crop seeds in two archaeobotanical studies, of the Islamic site of Khirbet Faris, S. Jordan and Neolithic sites in central Europe. Analyses using these functional measurements resulted in the identification of irrigation at the former and in the identification of sowing time and intensive cultivation at the latter. [Copyright &y& Elsevier]

Information about the topics discussed during the celebration of the Department of Health's National Breastfeeding Awareness Week (NBAW) on May 11, 2009 in London, England is presented. The event highlights the launch of the United Kingdom (UK)-World Health Organization (WHO) 0-4 years charts and focused on the discussion of how breastfeeding affects growth. It notes that the conference was hosted by Dr. Phil Hammond, a broadcaster and general practitioner (GP).

Abstract: Bovine digital dermatitis (BDD) is an epidermitis which is a leading cause of infectious lameness. The only recognized diagnostic test is foot inspection, which is a labour-intensive procedure. There is no universally recognized, standardized lesion scoring system. As small lesions are easily missed, foot inspection has limited diagnostic sensitivity. Furthermore, interpretation is subjective, and prone to observer bias. Serology is more convenient to carry out and is potentially a more sensitive indicator of infection. By carrying out 20 serological assays using lesion-associated Treponema spp. isolates, three serogroups were identified. The reliability of the tests was established by assessing the level of agreement and the concordance correlation coefficient. Subsequently, an ELISA suitable for routine use was developed. The benchmark of diagnostic test validation is conventionally the determination of the key test parameters, sensitivity and specificity. This requires the imposition of a cut-off point. For serological assays with outcomes on a continuous scale, the degree by which the test result differs from this cut-off is disregarded. Bayesian statistical methodology has been developed which enables the assay result also to be interpreted on a continuous scale, thereby optimizing the information inherent in the test. Using a cross-sectional study dataset carried out on 8 representative dairy farms in the UK, the probability of infection, P(I), of each individual animal was estimated in the absence of a ‘Gold Standard’ by modelling I as a latent variable which was determined by lesion status, L as well as serology, S. Covariate data (foot hygiene score and age) were utilized to estimate P(L) when no lesion inspection was performed. Informative prior distributions were elicited where possible. The model was utilized for predictive inference, by computing estimates of P(I) and P(L) independently of the data. A more detailed and informative analysis of the farm-level distribution of infection could thus be performed. Also, biases associated with the subjective interpretation of lesion status were minimized. Model outputs showed that young stock were unlikely to be infected, whereas cows tended to have high or low probabilities of being infected. Estimates of probability of infection were considerably higher for animals with lesions than for those without. Associations were identified between both covariates and probability of infection in cows, but not in the young stock. Under the condition that the model assumptions are valid for the larger population, the results of this work can be generalized by predictive inference. [Copyright &y& Elsevier]

The value of various types of psychosocial support for people with cancer is now becoming well established. Typically the term 'psychosocial' includes: counselling and psychotherapy, cognitive behaviour therapy, education and information, and social support. The research literature sometimes fails to clarify the exact nature of the different approaches and their relative effi cacy. Inevitably, even within a specifi c type of therapeutic approach, there is variation owing to the professional background and skills of different practitioners.This article describes the relative contributions made by an art psychotherapist and a clinical psychologist working together in a cancer and palliative care service in Wales. The referrals come from the same sources and tend to be for similar types of problem. The assessment and formulation processes are also broadly similar. Interventions, however, are markedly different. These are described in some detail through case study examples. [ABSTRACT FROM AUTHOR]

Objective To assess the safety and efficacy of long-term use of long-acting GnRH agonist in women with chronic cyclical pelvic pain using immediate versus delayed add-back hormonal replacement therapy (HRT). Design A prospective randomised trial. Setting Reproductive and Developmental Medicine, Academic Unit, University Teaching Hospital and NHS Hospitals. Population Thirty-eight premenopausal women with chronic cyclical pelvic pain were recruited. Methods Women were given Zoladex 10.8 mg over 18 months and randomised to receive HRT (tibolone 2.5 mg) either immediately or after 6 months. Follow up was 12-month post-treatment. Main outcome measures Bone mineral density at 6 months, the end of treatment (18 months), and 12 months later, pain and quality of life. Results Women treated with immediate HRT add-back showed less bone mineral density loss at 6 months and less vasomotor symptoms compared with those who had delayed HRT add-back treatment. Long-term follow up showed both groups experienced equivalent bone mineral density loss. Pain and health-related quality-of-life assessment showed improvement in both groups but there was evidence of a return to baseline levels after ending treatment. Conclusion Long-term use of GnRH agonist plus immediate add-back HRT is a safe and acceptable approach to intractable cyclical pelvic pain. Given the delay in reactivation of the hypothalamo-pituitary-ovarian axis after long-term suppression, an intermittent dose regime with GnRH agonist might warrant investigation. [ABSTRACT FROM AUTHOR]

The reproductive potential of overwintering boll weevil, Anthonomus grandis grandis (Boheman), females collected from pheromone traps in September, November and January, fed for 1, 3, and 5 weeks on plant pollens, and then provided cotton squares, was determined in the laboratory at 27 ± 1°C, 65% RH, and a photoperiod 13 : 11 (L : D) h. Duration of pollen feeding by overwintering boll weevils did not significantly influence egg and feeding punctures, or puncture ratios (egg to total punctures) for any of the three months of parent weevil collections when provided cotton squares on a daily basis. However, punctures and puncture ratios are significantly different when comparing mean data between months of boll weevil collections. When boll weevils were provided with cotton squares daily, the pre-ovipositional periods of female parents captured in September, November and January were 5, 9 and 14 days, respectively. The rate of eggs by females was significantly lower during November and January than September. Female parents collected in September produced a significantly higher percentage of eggs yielding adult progeny than those collected in November and January. Life table parameters indicated that net reproductive rate ( Ro) of boll weevil females collected in September was 1.2-fold higher than those collected in November and 10.7-fold higher than those collected in January. Except for testes size, no differences in male reproductive parameters were observed during the cotton-free period compared with males captured during mid-cotton (June). The number of oocytes in the ovarioles and the number of oocytes containing yolk were significantly lower during September, November and January compared with June. The reproductive potential of overwintering boll weevil females collected in different months is an important consideration in determining the success of any control strategy. [ABSTRACT FROM AUTHOR]

The axisymmetric buckling of a spherical shell embedded in an elastic medium with uniaxial compression at infinity is examined in the limit of small shell thickness ratio. An asymptotic method is developed by considering the paradigm problem of a beam attached to a Winkler substrate of variable stiffness, which in the small aspect ratio limit displays the same behavior as the shell. The asymptotic method is then applied to the Euler-Lagrange equations corresponding to shell buckling. The system is analyzed in two distinguished limits, displaying good agreement with the full numerical results. [ABSTRACT FROM AUTHOR]

Abstract: We present a robust and automatic method for evaluating the accuracy of Crop/Weed discrimination algorithms. The proposed method is based on simulated agronomic images and a Crop/inter-row Weed discrimination algorithm can be divided into the two following steps. Firstly a crop row detection (Hough transform) is performed from the identification of the crop line vanishing point taking the opportunity of the perspective geometry of the scene. Afterwards, the discrimination between crop and weeds is done by a region-based segmentation method using a blob-colouring analysis and an inter-row Weed Infestation Rate (WIR) can be estimated. We propose to test and validate the robustness of this method on simulated images with perspective. To simulate photos taken from a virtual camera, a pinhole camera model is used and the field is modelled according to the spatial periodicity distribution of crop seedlings and the spatial distribution of weed species based on stochastic processes (Poisson process, Neyman–Scott aggregative process or a mixture of both). For each simulated image, the comparison between the initial inter-row WIR and the detected inter-row WIR informs us about the errors made by the algorithm. A pixel classification between the two classes – Crop and Weed – is performed in order to identify misclassification errors. This comparison demonstrates an accuracy of better than 85% is possible for inter-row weed detection. [Copyright &y& Elsevier]

Background and Aims: This paper describes the changes in temperature-based indices used to classify viticultural climates in Australia for three warming scenarios produced by the Commonwealth Scientific and Industrial Research Organisation: Mk3.0 global climate model for the years 2030, 2050 and 2070. Methods and Results: Temperature indices that describe grapevine growing season temperature (GST), ripening period temperature, accumulated biologically effective degree days and growing season length were calculated to produce maps of Australia for each warming scenario. Summary statistics of each index's median and range are presented for each Australian wine region under each warming scenario. The greatest change in GST (above the 1971–2000 mean) was modelled to occur for the Perth Hills region, increasing by 1.0°C by 2030, 1.9°C by 2050 and 2.7°C by 2070. The least change in GST was modelled to occur for the Kangaroo Island region, increasing by 0.5°C by 2030, 0.9°C by 2050 and 1.3°C by 2070. Conclusion: Of the 61 recognised wine regions, a median GST of over 21°C (an indicator of the limit of quality wine grape production conditions) was found for three regions for the period 1971–2000, for eight regions for the 2030 scenario, 12 regions for the 2050 scenario and 21 regions for the 2070 scenario. Significance of the Study: Without appropriate adaptations, some established viticultural regions of Australia may become less suitable for quality winegrape production, whereas regions that were once considered unsuitable for quality winegrape production may become more suitable. [ABSTRACT FROM AUTHOR]

Midbrain dopamine (DA) neurons fire in 2 characteristic modes, tonic and phasic, which are thought to modulate distinct aspects of behavior. However, the inability to selectively disrupt these patterns of activity has hampered the precise definition of the function of these modes of signaling. Here, we addressed the role of phasic DA in learning and other DA-dependent behaviors by attenuating DA neuron burst firing and subsequent DA release, without altering tonic neural activity. Disruption of phasic DA was achieved by selective genetic inactivation of NMDA-type, ionotropic glutamate receptors in DA neurons. Disruption of phasic DA neuron activity impaired the acquisition of numerous conditioned behavioral responses, and dramatically attenuated learning about cues that predicted rewarding and aversive events while leaving many other DA-dependent behaviors unaffected. [ABSTRACT FROM AUTHOR]

The application of spatially explicit models of population dynamics to fisheries management and the design marine reserve network systems has been limited due to a lack of empirical estimates of larval dispersal. Here we compared assignment tests and parentage analysis for examining larval retention and connectivity under two different gene flow scenarios using panda clownfish ( Amphiprion polymnus) in Papua New Guinea. A metapopulation of panda clownfish in Bootless Bay with little or no genetic differentiation among five spatially discrete locations separated by 2–6 km provided the high gene flow scenario. The low gene flow scenario compared the Bootless Bay metapopulation with a genetically distinct population ( FST = 0.1) located at Schumann Island, New Britain, 1500 km to the northeast. We used assignment tests and parentage analysis based on microsatellite DNA data to identify natal origins of 177 juveniles in Bootless Bay and 73 juveniles at Schumann Island. At low rates of gene flow, assignment tests correctly classified juveniles to their source population. On the other hand, parentage analysis led to an overestimate of self-recruitment within the two populations due to the significant deviation from panmixia when both populations were pooled. At high gene flow (within Bootless Bay), assignment tests underestimated self-recruitment and connectivity among subpopulations, and grossly overestimated self-recruitment within the overall metapopulation. However, the assignment tests did identify immigrants from distant (genetically distinct) populations. Parentage analysis clearly provided the most accurate estimates of connectivity in situations of high gene flow. [ABSTRACT FROM AUTHOR]

Abstract: Herein we present results from experimental and theoretical studies concerning low temperature oxidation of CH4 over TiO2 supported Au nanoparticles. Our findings suggest that partial oxidation cannot be achieved under these conditions (1bar, 30–250°C). In order to understand this further, results from CO and H2 oxidation studies are also presented. The reaction rate for CH4 oxidation is found to be far lower than the rate for both CO and H2 oxidation, this goes towards explaining the strong tendency for total CH4 oxidation. These findings are further corroborated by DFT-calculations investigating the thermodynamics of CH4 oxidation on a stepped Au(211) surface. [Copyright &y& Elsevier]

This study examined the toxicological and physiological responses of a commercially important coral-reef grouper, Plectropomus leopardus (Serranidae), to injection of enriched stable-isotope barium chloride (BaCl2) solution. Thirty adult P. leopardus were subject to one of two 138BaCl2 injection treatment groups (corresponding to dosage rates of 2 and 4 mg 138Ba kg−1 body mass), and a control group in which fish were injected with 0·9% sodium chloride (NaCl) solution. Fish from each group were sampled at post-injection intervals of 48 h and 1, 3, 5 and 8 weeks, at which time blood and tissue samples were removed from each fish. Residual concentrations of Ba and 138Ba:137Ba ratios were measured in muscle, gonad, liver and bone tissues of each experimental fish. Elevated Ba concentrations were detected in all treatment fish tissue samples within 48 h post injection. Residual Ba concentrations decreased throughout the remainder of the 8 week experimental period in all tissues except bone. The BaCl2 injection had no significant effects on measured whole blood variables or on the plasma concentrations of steroid hormones. Enriched Ba stable isotopes can therefore be used at low dosages to mark larvae of commercially important marine fishes, without adverse effects on the health of the fishes or on humans who may consume them. [ABSTRACT FROM AUTHOR]

The problem of a thin spherical linearly elastic shell perfectly bonded to an infinite linearly elastic medium is considered. A constant axisymmetric stress field is applied at infinity in the matrix, and the displacement and stress fields in the shell and matrix are evaluated by means of harmonic potential functions. In order to examine the stability of this solution, the buckling problem of a shell which experiences this deformation is considered. Using Koiter's nonlinear shallow shell theory, restricting buckling patterns to those which are axisymmetric and using the Rayleigh–Ritz method by expanding the buckling patterns in an infinite series of Legendre functions, an eigenvalue problem for the coefficients in the infinite series is determined. This system is truncated and solved numerically in order to analyse the behaviour of the shell as it undergoes buckling and to identify the critical buckling stress in two cases, namely, where the shell is hollow and the stress at infinity is either uniaxial or radial. [ABSTRACT FROM PUBLISHER]

Bushclumps are scattered islands of thicket-like vegetation within a matrix of more open vegetation. We investigated the role of bushclumps as refugia for small mammals, and examined the effect of a limited number of abiotic and biotic factors on their richness, diversity and abundance. Small mammals were surveyed using Sherman small mammal traps at two sites in the Eastern Cape Province, South Africa (Mountain Zebra National Park and Kwandwe Private Game Reserve). Soil hardness and seed abundance, inside and outside bushclumps, were determined. Trap success was significantly higher inside bushclumps than in areas outside, and species diversity and the abundance of small mammals were greater within bushclumps compared to outside. Bushclumps also had significantly softer soil and a higher concentration of seeds. We conclude that bushclumps provide a concentrated source of food and protection from predators for small mammals, and are thus used significantly more than adjacent open areas. The conservation of bushclumps is therefore important for the overall maintenance of ecosystem functioning. [ABSTRACT FROM AUTHOR]

Objectives: To determine the prevalence and nature of rock-climbing injuries, and the factors associated with these injuries. Design: A retrospective cross-sectional study. Setting: Rock climbers were recruited at five outdoor and six indoor climbing venues in the UK. Participants: 201 active rock climbers (163 male, 38 female climbers) aged 16-62 years. Assessment of risk factors: Rock climbing behaviours and key demographics. Main outcome measures: Injuries requiring medical attention or withdrawal from participation for ⩾1 day. Results: Around 50% of climbers had sustained ⩾1 injury in the past 12 months, causing a total of 275 distinct anatomical injuries. 21 climbers (10%) had sustained acute climbing injuries as a result of a fall, 67(33%) had chronic overuse injuries, and 57(28%) had acute injuries caused by strenuous climbing moves. Dedicated climbers participating in different forms of rock climbing more often and at a higher level of technical difficulty may be more prone to injury, particularly overuse injuries of the finger and shoulder. The principal sources of treatment or advice sought by climbers were physiotherapists (18%), other climbers (14%) and doctors (11%). Conclusions: Climbing frequency and technical difficulty are associated with climbing injuries occurring at both indoor and outdoor venues, particularly cumulative trauma to the upper extremities. [ABSTRACT FROM AUTHOR]

In recent years there has been an increasing demand for the design, development and use of questionnaires that can assess patients' experiences of health and illness or their health-related quality of life (HRQoL). The application of these questionnaires in research and clinical practice provides information from the patient's perspective on the ways in which symptoms and treatments can affect their daily well-being and functioning. The aim of this paper is to report on the ways in which information on the HRQoL of women with polycystic ovary syndrome (PCOS) can be collected. With particular reference to PCOS-associated infertility, this paper will discuss the types of questionnaires that are currently available. It will discuss the benefits and limitations of the disease-specific PCOS questionnaire, and the other generic and condition-specific tools that have been used in the existing PCOS literature. Recommendations for future researchers interested in measuring HRQoL outcomes in women with PCOS-associated infertility are also made. [ABSTRACT FROM AUTHOR]

This chapter will review what is known about the charging of planetary rings, in particular the sum of the individual currents from the time-varying charge dQ/ dt, of the planetary ring particle. For the smallest ring particles, in addition to checking the plasma conditions for the charging currents, one must consider if collective effects in the ring environment are relevant. Two planetary ring environments that have held a strong interest for ring scientists in the last two decades are Saturn’s spokes in the B Ring and the environment of Saturn’s E ring. Two sections of this chapter will describe these planetary ring charging environments in detail. Finally, we describe two charging effects that demonstrate areas of future studies while providing fresh examples of the intriguing effects from planetary ring charging processes. [ABSTRACT FROM AUTHOR]

Light is an important environmental factor for regulation of mood. There is a high frequency of seasonal affective disorder in high latitudes where light exposure is limited, and bright light therapy is a successful antidepressant treatment. We recently showed that rats kept for 6 weeks in constant darkness (DD) have anatomical and behavioral features similar to depressed patients, including dysregulation of circadian sleep-waking rhythms and impairment of the noradrenergic (NA)-locus coeruleus (LC) system. Here, we analyzed the cell viability of neural systems related to the pathophysiology of depression after DD, including NA-IC, serotoninergic-raphe nuclei and dopaminergic-ventral tegmental area neurons, and evaluated the depressive behavioral profile of light-deprived rats. We found increased apoptosis in the three aminergic systems analyzed when compared with animals maintained for 6 weeks in 12:12 light-dark conditions. The most apoptosis was observed in NA-LC neurons, associated with a significant decrease in the number of cortical NA boutons. Behaviorally, DD induced a depression-like condition as measured by increased immobility in a forced swim test (FST). DD did not appear to be stressful (no effect on adrenal or body weights) but may have sensitized responses to subsequent stressors (increased fecal number during the FST). We also found that the antidepressant desipramine decreases these neural and behavioral effects of light deprivation. These findings indicate that DD induces neural damage in monoamine brain systems and this damage is associated with a depressive behavioral phenotype. Our results suggest a mechanism whereby prolonged limited light intensity could negatively impact mood. [ABSTRACT FROM AUTHOR]

Saturn's moon Rhea had been considered massive enough to retain a thin, externally generated atmosphere capable of locally affecting Saturn's magnetosphere. The Cassini spacecraft's in situ observations reveal that energetic electrons are depleted in the moon's vicinity. The absence of a substantial exosphere implies that Rhea's magnetospheric interaction region, rather than being exclusively induced by sputtered gas and its products, likely contains solid material that can absorb magnetospheric particles. Combined observations from several instruments suggest that this material is in the form of grains and boulders up to several decimetres in size and orbits Rhea as an equatorial debris disk. Within this disk may reside denser, discrete rings or arcs of material. [ABSTRACT FROM AUTHOR]

Sex-biased behaviours are expected to play an important role in partitioning genetic variance in animal populations. Comparing genetic structure at markers with different modes of inheritance provides a means of detecting these behaviours and their consequences for population genetic structure. In colonially breeding mammals, the common combination of female philopatry and male vagility can promote contrasting patterns of genetic differentiation between the sexes, both via their effects on recurrent gene flow and on colonization. We examined sex differences in gene flow and structure by comparing maternally inherited mitochondrial DNA (mtDNA) and biparentally inherited autosomal loci in the Formosan lesser horseshoe bat Rhinolophus monoceros. We found that genetic partitioning was higher at mtDNA than autosomal markers in both sexes, indicative of female-biased philopatry and male-biased dispersal. Across Taiwan, isolation-by-distance was detected for all sex/marker combinations but was steeper for mtDNA than for nuclear markers. We suggest that isolation-by-distance shown from mtDNA at large scales is likely to reflect the stepwise founding of new breeding colonies by females during colonization. In contrast, no isolation-by-distance was found at smaller distances of up to 100 km, indicating that gene flow and/or recent shared ancestry homogenises genetic structure among nearby sites. Our results highlight the value of an indirect genetic approach to understanding sex-biased behaviours and their consequences in a little-studied species. [ABSTRACT FROM AUTHOR]

Acoustic emission (AE) monitoring is a non-invasive method of monitoring fracturing both in situ, and in experimental rock deformation studies. Until recently, the major impediment for imaging brittle failure within a rock mass is the accuracy at which the hypocenters may be located. However, recent advances in the location of regional scale earthquakes have successfully reduced hypocentral uncertainties by an order of magnitude. The least-squares Geiger, master event relocation, and double difference methods have been considered in a series of synthetic experiments which investigate their ability to resolve AE hypocentral locations. The effect of AE hypocenter location accuracy due to seismic velocity perturbations, uncertainty in the first arrival pick, array geometry and the inversion of a seismically anisotropic structure with an isotropic velocity model were tested. Hypocenters determined using the Geiger procedure for a homogeneous, isotropic sample with a known velocity model gave a RMS error for the hypocenter locations of 2.6 mm; in contrast the double difference method is capable of reducing the location error of these hypocenters by an order of magnitude. We test uncertainties in velocity model of up to ±10% and show that the double difference method can attain the same RMS error as using the standard Geiger procedure with a known velocity model. The double difference method is also capable of precise locations even in a 40% anisotropic velocity structure using an isotropic model for location and attains a RMS mislocation error of 2.6 mm that is comparable to a RMS mislocation error produced with an isotropic known velocity model using the Geiger approach. We test the effect of sensor geometry on location accuracy and find that, even when sensors are missing, the double difference method is capable of a 1.43 mm total RMS mislocation compared to 4.58 mm for the Geiger method. The accuracy of automatic picking algorithms used for AE studies is ±0.5 µs (1 time sample when the sampling rate is 0.2 µs). We investigate how AE locations are effected by the accuracy of first arrival picking by randomly delaying the actual first arrival by up to 5 time samples. We find that even when noise levels are set to 5 time samples the double difference method successfully relocates the synthetic AE. [ABSTRACT FROM AUTHOR]

The ATP-binding cassette A1 (ABCA1) protein regulates plasma high-density lipoprotein (HDL) levels. Mutations in ABCA1 can cause HDL deficiency and increase the risk of premature coronary artery disease. Single nucleotide polymorphisms (SNPs) in ABCA1 are associated with variation in plasma HDL levels. We investigated the prevalence of mutations and common SNPs in ABCA1 in 154 low-HDL individuals and 102 high-HDL individuals. Mutations were identified in five of the low-HDL subjects, three having novel variants (I659V, R2004K, and A2028V) and two with a previously identified variant (R1068H). Analysis of four SNPs in the ABCA1 gene promoter (C-564T, G-407C, G-278C, and C-14T) identified the C-14T SNP and the TCCT haplotype to be over-represented in low-HDL individuals. The R1587K SNP was over-represented in low-HDL individuals, and the V825I and I883M SNPs over-represented in high-HDL individuals. We conclude that sequence variation in ABCA1 contributes significantly to variation in HDL levels. [ABSTRACT FROM AUTHOR]