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Inorder to analyze flavonoids in pollen, stem and leaf of Typha angustifolia and the contents of typhaneoside, isorhamnetin-3-O-neohesperidoside and isorhamnetin in different parts of growth periods. UPLC-Q-TOF-MS was used for qualitative analysis, combined with HPLC to analyze and evaluate the contents of flavonoids in different parts of T. angustifolia. A RPAZIS C18 column(250 mm × 4.6 mm, 5 μm)was adopted. The mobile phase was composed of acetonitrile-0.05% phosphoric acid with a gradient elution. The flow rate was 1.0 mL·min-1, the detection wavelength was 254 nm, and the column temperature was 30 ℃. The results were as follows:(1)A total of 42 compounds were identified in pollen, stem and leaf, and eight compounds with great differences in different parts.(2)The contents of three flavonoids in different parts of T. angustifolia during different growth periods in the same growth environment were significantly different.(3)The contents of isorhamnetin-3-O-neohesperidoside and isorhamnetin in the plant were increased first and then decreased, while decreased significantly in the stem and leaf; In pollen, the contents of typhaneoside and isorhamnetin-3-O-neohesperidoside increased quickly and reached a peak within a week; The contents of isorhamnetin in stem and leaf were low and were not detected in most samples. This established method in this study was simple and reproducible. The changes regularity of the flavonoids content in T. angustifolia had been studied and the differences of compounds in pollen, stem and leaf were also analyzed. This research provides a scientific basis for reasonable resource utilization of T. angustifolia [ABSTRACT FROM AUTHOR]